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Invitrogen
AgRP Polyclonal Antibody
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FIGURE: 1 / 1
AgRP Antibody (PA1-18414) in IHC (P)
Immunohistochemistry was performed on 4% paraformaldehyde-fixed paraffin-embedded mouse brain tissue sections. To expose target antigens, tissues were treated with 15 µg/mL Proteinase K in PBS containing 0.01% Triton X-100 for 5 minutes. Tissues were blocked for 1 hour and probed with an Agouti Related Protein polyclonal antibody (Product # PA1-18414) at a dilution of 1:200 overnight at 4°C. Tissues were wa... View More
Product Details
PA1-18414
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Sheep,
Rat
Published species
Mouse
Host/Isotype
Guinea pig
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region (82-131) within the carboxy domain of mouse agouti related protein conjugated to carrier protein.
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute in 50 µL sterile water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8 °C. It is recommended that a thawed sample is stored at 4 °C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20 °C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Target Information
Agouti-related protein (AgRP), also called agouti-related peptide, is a neuropeptide produced in the brain by the AgRP/NPY neuron. In humans, the agouti-related peptide is encoded by the AGRP gene. This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: agouti related protein; agouti related protein homolog; Agouti-related protein; orexigenic neuropeptide
Gene Aliases: AGRP; AGRT; ART; ASIP2
UniProt ID: (Human) O00253, (Mouse) P56473
Entrez Gene ID: (Sheep) 443269, (Human) 181, (Mouse) 11604, (Rat) 25582
neuropeptide signaling pathway
circadian rhythm
feeding behavior
adult feeding behavior
hormone-mediated signaling pathway
response to insulin
eating behavior
long-day photoperiodism
maternal process involved in female pregnancy
regulation of feeding behavior
positive regulation of feeding behavior
photoperiodism
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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