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Invitrogen
Phospho-FAK (Ser910) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-FAK (Ser910) Antibody (PA1-28423) in WB
Western blot detection of Phospho-FAK pSer910 in extracts of human epithelial carcinoma cells expressing FAK with (lanes 2-5) or without (lane 1) 100 ng/mL Taxol for 18-24 hours in serum-free media to arrest cells in mitosis. Samples were resolved by SDS-PAGE on a 10% Tris-glycine gel, transferred to PVDF and incubated using a Phospho-FAK pSer910 polyclonal antibody (Product # PA1-28423).
Product Details
PA1-28423
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the region of FAK that contains serine 910. The sequence is conserved in human, mouse, rat, chicken and frog.
Conjugate
Form
Liquid
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
PA1-28423 detects FAK from human samples.
PA1-28423 has been successfully used in Western blot applications.
The PA1-28423 immunogen is: Synthetic peptide derived from the region of FAK that contains serine 910. The sequence is conserved in human, mouse, rat, chicken and frog.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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