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Invitrogen
ERO1L Polyclonal Antibody
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Product Details
PA1-46120
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide made to the N-terminal region of human ERO1L.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1% BSA
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This ERO1L antibody is useful for Western Blot, where a band ~57 kDa is observed.
Depending on the samples used ERO1L can also appear as a doublet which is believed to represent the oxidized and reduced forms of the protein.
Suggested positive control: 293T, INS-1, and MIN6 cell lysate.
Target Information
ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in ER membrane as peripheral membrane protein on the lumenal side. Its association with ERP44 is essential for its retention in the ER and is unique in coupling oxygen reduction to de novo disulfides formation. The ERO1L enzyme is broadly distributed at low levels in several tissues with highest levels in the upper digestive tract and is stimulated by hypoxia via HIF-signaling. ERO1L generally exists as monomer, but has the ability to function as monomer or homodimer and interacts with PDILT. After translation, ERO1L gets n-glycosylated and the disulfide bonds constitute the redox-active center whereby the Cys-94/Cys-99 disulfide bond accepts an electron from P4HB followed by funneling the same to the active site disulfide Cys-394/Cys-397. ERO1L acts by oxidizing directly to P4HB/PDI isomerase through direct disulfide exchange and associates with ERP44. ERO1L is implicated in immunoglobulin folding, oxidative stress, release of unfolded cholera toxin from reduced P4HB/PDI upon Vibrio cholerae infection, and plays a key role in ER stress-induced CHOP-dependent apoptosis through inositol 1,4,5-trisphosphate receptor IP3R1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Endoplasmic oxidoreductin-1-like protein; endoplasmic reticulum oxidation 1; Endoplasmic reticulum oxidoreductase alpha; Endoplasmic reticulum oxidoreductin-1-like protein; ero1; ERO1 homolog; ERO1-L; ERO1-L-alpha; ERO1-like protein alpha; Global ischemia-induced protein 11; Oxidoreductin-1-L-alpha; unnamed protein product
Gene Aliases: ERO1-alpha; ERO1-L; ERO1-L-alpha; ERO1A; Ero1alpha; ERO1L; ERO1LA; Giig11; UNQ434/PRO865
UniProt ID: (Human) Q96HE7, (Mouse) Q8R180, (Rat) Q8R4A1
Entrez Gene ID: (Human) 30001, (Mouse) 50527, (Rat) 171562
protein binding
protein disulfide oxidoreductase activity
oxidoreductase activity
thiol oxidase activity
FAD binding
protein disulfide isomerase activity
peptide-methionine (S)-S-oxide reductase activity
oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
peptide-methionine (R)-S-oxide reductase activity
methionine-R-sulfoxide reductase activity
oxidoreductase
protein folding
apoptotic process
response to oxidative stress
transforming growth factor beta receptor signaling pathway
skeletal muscle tissue development
response to temperature stimulus
L-ascorbic acid metabolic process
extracellular matrix organization
collagen fibril organization
endoplasmic reticulum unfolded protein response
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
protein modification process
cell redox homeostasis
brown fat cell differentiation
release of sequestered calcium ion into cytosol
protein maturation
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hypoxia
transport
4-hydroxyproline metabolic process
protein maturation by protein folding
chaperone mediated protein folding requiring cofactor
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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