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Invitrogen
NuMA Polyclonal Antibody
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FIGURE: 1 / 18
NuMA Antibody (PA3-16829) in ICC/IF
Immunofluorescence analysis of NuMA was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NuMA Polyclonal Antibody (Product # PA3-16829) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a diluti... View More
Product Details
PA3-16829
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mammal,
Marsupial,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: Hela whole cell extract.
Target Information
NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen; structural nuclear protein; unnamed protein product
Gene Aliases: 6720401E04Rik; AA764025; AL022610; AU014979; NMP-22; NMP22; NUMA; NUMA1
UniProt ID: (Human) Q14980, (Mouse) E9Q7G0
Entrez Gene ID: (Human) 4926, (Rat) 308870, (Mouse) 101706
structural molecule activity
protein binding
microtubule binding
lipid binding
tubulin binding
protein domain specific binding
phosphatidylinositol binding
macromolecular complex binding
microtubule plus-end binding
microtubule minus-end binding
dynein complex binding
disordered domain specific binding
phosphorelay sensor kinase activity
cytoskeletal protein
microtubule or microtubule-binding cytoskeletal protein
establishment of mitotic spindle orientation
microtubule bundle formation
nucleus organization
chromosome segregation
positive regulation of BMP signaling pathway
astral microtubule organization
positive regulation of microtubule polymerization
positive regulation of intracellular transport
positive regulation of keratinocyte differentiation
cell division
meiotic cell cycle
positive regulation of hair follicle development
positive regulation of chromosome segregation
anastral spindle assembly
regulation of mitotic spindle organization
regulation of metaphase plate congression
positive regulation of protein localization to spindle pole body
positive regulation of mitotic spindle elongation
positive regulation of protein localization to cell cortex
positive regulation of chromosome separation
positive regulation of spindle assembly
phosphorelay signal transduction system
signal transduction by protein phosphorylation
lung epithelial cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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