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Invitrogen
GCP2 Polyclonal Antibody
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FIGURE: 1 / 5
GCP2 Antibody (PA5-103851) in ICC/IF
Immunofluorescent analysis of GCP2 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GCP2 polyclonal antibody (Product # PA5-103851) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-103851
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TUBGCP2(Accession Q9BSJ2), corresponding to amino acid residues R540-L590.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TUBGCP2.
Target Information
The γ-Tubulin complex is composed of γ Tubulin and the γ-Tubulin complexassociated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. γ-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the γ-Tubulin complex. GCP2 (γ-Tubulin complex component 2), also known as TUBGCP2 or SPBC97 (spindle pole body protein Spc97 homolog) is a ubiquitously expressed 902 amino acid protein that localizes to the centrosome and is involved in microtubule nucleation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a disintegrin and metalloprotease domain 8; component of cytoplasmic gamma-tubulin complex; Gamma-ring complex protein 103 kDa; Gamma-tubulin complex component 2; GCP-2; h103p; hGrip103; hSpc97; Spindle pole body protein Spc97 homolog; tubulin gamma complex associated protein 2; unnamed protein product
Gene Aliases: 1700022B05Rik; Adam8; ALP4; GCP-2; GCP2; Grip103; h103p; hGCP2; hSpc97; PAMDDFS; SPBC97; SPC97; Spc97p; TUBGCP2
UniProt ID: (Human) Q9BSJ2, (Mouse) Q921G8
Entrez Gene ID: (Human) 10844, (Mouse) 74237, (Rat) 309098
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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