Search
Invitrogen
Phospho-SREBP1 (Ser396) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
Phospho-SREBP1 (Ser396) Antibody (PA5-105169) in WB
Western blot analysis of Phospho-SREBP1 (Ser396) in extracts from RAW264.7 cells (serum starvation treatment). Samples were incubated with Phospho-SREBP1 (Ser396) polyclonal antibody (Product # PA5-105169). The lane on the left was treated with blocking peptide.
Product Details
PA5-105169
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SREBF1(Accession P36956), corresponding to amino acid residues around phosphorylated Ser396.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of SREBP1 only when phosphorylated at Ser396.
Target Information
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADD1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; SREBP-1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1; unnamed protein product
Gene Aliases: ADD-1; ADD1; BHLHD1; D630008H06; HMD; IFAP2; SREBF1; SREBP-1; SREBP-1a; SREBP-1c; SREBP1; SREBP1c
UniProt ID: (Human) P36956, (Rat) P56720, (Mouse) Q9WTN3
Entrez Gene ID: (Human) 6720, (Rat) 78968, (Mouse) 20787
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
protein kinase binding
sterol response element binding
sequence-specific DNA binding
macromolecular complex binding
protein dimerization activity
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein complex binding
transcription regulatory region DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of heart rate by chemical signal
transcription, DNA-templated
regulation of transcription, DNA-templated
aging
circadian rhythm
cholesterol metabolic process
insulin receptor signaling pathway
lipid biosynthetic process
cellular response to starvation
response to glucose
positive regulation of triglyceride biosynthetic process
response to organic cyclic compound
regulation of fatty acid metabolic process
lung development
positive regulation of histone deacetylation
response to food
response to retinoic acid
response to progesterone
response to insulin
cellular response to insulin stimulus
response to glucagon
response to lipid
response to drug
response to peptide hormone
positive regulation of cholesterol biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
regulation of insulin secretion
response to cAMP
response to fatty acid
cellular response to fatty acid
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
lipid metabolic process
cholesterol biosynthetic process
response to nutrient
steroid metabolic process
response to xenobiotic stimulus
response to fructose
insulin secretion
intracellular receptor signaling pathway
regulation of protein stability
SREBP signaling pathway
mRNA transcription from RNA polymerase II promoter
fat cell differentiation
response to ethanol
negative regulation of triglyceride metabolic process
regulation of mitophagy
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of protein targeting to mitochondrion
positive regulation of fatty acid biosynthetic process
regulation of mitophagy
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support