Search
Invitrogen
Phospho-IRAK1 (Thr209) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 6
Phospho-IRAK1 (Thr209) Antibody (PA5-105183) in IHC (P)
Immunohistochemistry analysis of Phospho-IRAK1 (Thr209) in rat stomach tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-IRAK1 (Thr209) polyclonal antibody (Product # PA5-105183) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-105183
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IRAK1(Accession P51617), corresponding to amino acid residues around phosphorylated Thr209.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Application note: This antibody has also been used in peptide-ELISA
Antibody detects endogenous levels of IRAK1 only when phosphorylated at Thr209.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: hIRAK1c; protein kinase; inactive protein kinase; signaling molecule; Interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; IRAK-1; mPLK; Pelle homolog; Pelle-like protein kinase
Gene Aliases: AA408924; Il1rak; IRAK; IRAK-1; IRAK1; IRAK1-S; mPLK; pelle; Plpk; RGD1563841
UniProt ID: (Human) P51617, (Mouse) Q62406
Entrez Gene ID: (Human) 3654, (Mouse) 16179, (Rat) 363520
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
heat shock protein binding
identical protein binding
protein homodimerization activity
protein heterodimerization activity
protein serine kinase activity
interleukin-1 receptor binding
transferase activity, transferring phosphorus-containing groups
regulation of cytokine-mediated signaling pathway
toll-like receptor signaling pathway
immune system process
MyD88-dependent toll-like receptor signaling pathway
regulation of transcription, DNA-templated
signal transduction
I-kappaB kinase/NF-kappaB signaling
JNK cascade
Toll signaling pathway
positive regulation of macromolecule metabolic process
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
positive regulation of type I interferon production
response to lipopolysaccharide
toll-like receptor 2 signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 9 signaling pathway
cellular response to heat
intracellular signal transduction
interleukin-33-mediated signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
protein autophosphorylation
positive regulation of smooth muscle cell proliferation
positive regulation of NF-kappaB transcription factor activity
type I interferon signaling pathway
interleukin-1-mediated signaling pathway
response to interleukin-1
cellular response to lipopolysaccharide
cellular response to hypoxia
positive regulation of intracellular signal transduction
positive regulation of leukocyte adhesion to vascular endothelial cell
protein phosphorylation
activation of NF-kappaB-inducing kinase activity
phosphorylation
negative regulation of NF-kappaB transcription factor activity
response to peptidoglycan
positive regulation of MAP kinase activity
positive regulation of JUN kinase activity
negative regulation of transcription, DNA-templated
protein oligomerization
negative regulation of cholesterol efflux
aging
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support