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Invitrogen

Phospho-IKB alpha (Tyr42) Polyclonal Antibody

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Phospho-IKB alpha (Tyr42) Polyclonal Antibody

Product Details

PA5-105545

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NFKBIA(Accession P25963), corresponding to amino acid residues around phosphorylated Tyr42.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816973

Product Specific Information

Antibody detects endogenous levels of IKB alpha only when phosphorylated at Tyr42.

Target Information

IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: I(Kappa)B(alpha); I-kappa-B-alpha; I-kappaBalpha; IkappaBalpha; IkB-alpha; IkBa; IKBalpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; Mutations: 945:OK; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; RL/IF-1; unnamed protein product

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Gene Aliases: AI462015; EDAID2; IKBA; MAD-3; MAD3; NFKBI; NFKBIA; RL/IF-1

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UniProt ID: (Human) P25963, (Rat) Q63746, (Mouse) Q9Z1E3

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Entrez Gene ID: (Human) 4792, (Rat) 25493, (Mouse) 18035

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Function(s)
protein binding nuclear localization sequence binding enzyme binding ubiquitin protein ligase binding identical protein binding NF-kappaB binding protein sequestering activity transcription regulator inhibitor activity heat shock protein binding protein complex binding
Process(es)
protein import into nucleus, translocation cytoplasmic sequestering of NF-kappaB regulation of gene expression negative regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux negative regulation of lipid storage lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity positive regulation of cellular protein metabolic process response to muramyl dipeptide response to lipopolysaccharide toll-like receptor 4 signaling pathway response to muscle stretch regulation of cell proliferation cytoplasmic sequestering of transcription factor response to exogenous dsRNA negative regulation of myeloid cell differentiation negative regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter nucleotide-binding oligomerization domain containing 1 signaling pathway nucleotide-binding oligomerization domain containing 2 signaling pathway negative regulation of transcription from RNA polymerase II promoter immune system process regulation of transcription from RNA polymerase II promoter protein import into nucleus inflammatory response Notch signaling pathway I-kappaB kinase/NF-kappaB signaling signal transduction involved in regulation of gene expression negative regulation of cholesterol transport tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling negative regulation of protein import into nucleus negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of inflammatory response B cell receptor signaling pathway positive regulation of transcription initiation from RNA polymerase II promoter cellular response to cold interleukin-1-mediated signaling pathway cellular response to cytokine stimulus cellular response to tumor necrosis factor negative regulation of cytokine production involved in inflammatory response
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