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Invitrogen
RFWD2 Polyclonal Antibody
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FIGURE: 1 / 4
RFWD2 Antibody (PA5-106657) in IHC (P)
Immunohistochemistry analysis of RFWD2 in mouse muscle tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RFWD2 polyclonal antibody (Product # PA5-106657) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-106657
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RFWD2(Accession Q8NHY2), corresponding to amino acid residues T361-L411.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total RFWD2.
Target Information
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Constitutive photomorphogenesis protein 1 homolog; constitutive photomorphogenic protein 1; COP1D; E3 ubiquitin protein ligase; E3 ubiquitin-protein ligase COP1; E3 ubiquitin-protein ligase RFWD2; hCOP1; hCOP1 d24; mCOP1; putative ubiquitin ligase COP1; ring finger and WD repeat domain 2, E3 ubiquitin protein ligase; RING finger and WD repeat domain protein 2; RING finger protein 200; RING-finger protein; RING-type E3 ubiquitin transferase RFWD2; unnamed protein product
Gene Aliases: AI316802; C80879; CFAP78; COP1; FAP78; RFWD2; RGD1304773; RNF200
UniProt ID: (Human) Q8NHY2, (Mouse) Q9R1A8
Entrez Gene ID: (Human) 64326, (Mouse) 26374, (Rat) 360860
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
metal ion binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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