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Invitrogen
Phospho-PAK2 (Ser20) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-PAK2 (Ser20) Antibody (PA5-110143) in WB
Western blot analysis of Phospho-PAK2 (Ser20) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-PAK2 (Ser20) Polyclonal antibody (Product # PA5-110143) using a dilution of 1:2,000 or PAK2 antibody (Product # PA5-96641). Followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by Calyculin A (100 nM) for 30 minutes after serum-starvat... View More
Product Details
PA5-110143
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S20 of human PAK2 (NP_002568.2).
Conjugate
Form
Liquid
Concentration
0.53 mg/mL
Amount
53 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSSTI
Target Information
PAK2 (serine/threonine-protein kinase PAK2) is a serine/threonine kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, or proliferation. It acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformation change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-PAK; Beta-PAK; gamma pak; Gamma-PAK; Oligophrenin-3; OPHN3; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p58; p65-PAK; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK1; PAK3; PAK65; S6/H4 kinase; Serine/threonine-protein kinase PAK 2; unnamed protein product
Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; D16Ertd269e; KNO2; mKIAA4182; PAK-2; PAK2; PAK65; PAKgamma
UniProt ID: (Human) Q13177, (Mouse) Q8CIN4
Entrez Gene ID: (Human) 5062, (Mouse) 224105
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
protein tyrosine kinase activator activity
small GTPase binding
identical protein binding
cadherin binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
Rac GTPase binding
non-receptor serine/threonine protein kinase
stimulatory C-type lectin receptor signaling pathway
cardiac muscle hypertrophy
protein phosphorylation
negative regulation of protein kinase activity
apoptotic process
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
cellular response to starvation
cell migration
adherens junction assembly
intracellular signal transduction
negative regulation of apoptotic process
regulation of MAPK cascade
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
regulation of axonogenesis
regulation of cytoskeleton organization
negative regulation of stress fiber assembly
protein maturation
dendritic spine development
bicellular tight junction assembly
cellular response to transforming growth factor beta stimulus
execution phase of apoptosis
regulation of actin filament organization
protein localization to cell-cell junction
positive regulation of execution phase of apoptosis
positive regulation of extrinsic apoptotic signaling pathway
Rho protein signal transduction
regulation of mitotic cell cycle
metabolic process
phosphorylation
peptidyl-serine phosphorylation
signal transduction by protein phosphorylation
actin cytoskeleton organization
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of growth
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of protein tyrosine kinase activity
cellular response to organic cyclic compound
negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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