Search
Invitrogen
Phospho-BCLAF1 (Ser531) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 6
Phospho-BCLAF1 (Ser531) Antibody (PA5-118678) in ICC/IF
Immunocytochemistry analysis of Phospho-BCLAF1 (Ser531) in Hela cells (Forskolin 500 μm for 30 min). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-BCLAF1 (Ser531) polyclonal antibody (Product # PA5-118678) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat an... View More
Product Details
PA5-118678
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BCLAF1(Accession Q9NYF8), corresponding to amino acid residues around phosphorylated Ser531.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of BCLAF1 only when phosphorylated at Ser531.
Target Information
BCLAF1 is an intracellular, death promoting transcriptional repressor of survival genes that interacts with several members of the BCL2 family of proteins. It shows homology to the basic leucine zipper (bzip) and MYB DNA binding domains.From recent research it can be concluded that in case of apoptotic cells this protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope. When over expressed this protein induces apoptosis and can be suppressed by co-expression of BCL2 proteins. Also known as BTF, it triggers a proapoptotic signal, potentially helpful to overcome the resistance to STI571.BCLAF1 is found to express mostly in skeletal muscles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Bcl-2-associated transcription factor 1; BCLAF1 and THRAP3 family member 1; BP-1; Btf; Btf-s; KIAA0164; unnamed protein product
Gene Aliases: 2610102K23Rik; 2700025J07Rik; 2810454G14Rik; 5730534O06Rik; Aa2-041; AI450190; AW556225; BCLAF1; bK211L9.1; BTF; KIAA0164; mKIAA0164
UniProt ID: (Human) Q9NYF8, (Mouse) Q8K019
Entrez Gene ID: (Human) 9774, (Mouse) 72567, (Rat) 293017
apoptotic process
cellular response to DNA damage stimulus
positive regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to leukemia inhibitory factor
positive regulation of DNA-templated transcription, initiation
positive regulation of intrinsic apoptotic signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of DNA-templated transcription in response to stress
positive regulation of response to DNA damage stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support