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Invitrogen
ITGB5 Polyclonal Antibody
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FIGURE: 1 / 1
ITGB5 Antibody (PA5-120815) in WB
Western blot analysis of ITGB5 in extracts of various cell lines. Samples were incubated with ITGB5 Polyclonal antibody (Product # PA5-120815) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-120815
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 490-720 of human ITGB5 (NP_002204.2).
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Amount
42 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: Mouse brain, Mouse kidney, Mouse liver, Rat brain, Rat liver. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein.
Immunogen sequence: SPGYLGTRCE CQDGENQSVY QNLCREAEGK PLCSGRGDCS CNQCSCFESE FGKIYGPFCE CDNFSCARNK GVLCSGHGEC HCGECKCHAG YIGDNCNCST DISTCRGRDG QICSERGHCL CGQCQCTEPG AFGEMCEKCP TCPDACSTKR DCVECLLLHS GKPDNQTCHS LCRDEVITWV DTIVKDDQEA VLCFYKTAKD CVMMFTYVEL PSGKSNLTVL REPECGNTPN A
Target Information
Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD61; FLJ26658; GP3A; GPIIIa; Integrin beta-5; integrin beta-5 subunit; integrin beta-5 subunit precursor; testis secretory sperm-binding protein Li 217p; unnamed protein product
Gene Aliases: [b]-5; [b]5; [b]5A; [b]5B; AA475909; AI874634; beta-5; beta5; ESTM23; ITGB5; RGD1563276
UniProt ID: (Human) P18084, (Mouse) O70309
Entrez Gene ID: (Human) 3693, (Rat) 257645, (Mouse) 16419
formation of primary germ layer
cell adhesion
cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
cell migration
cell adhesion mediated by integrin
wound healing, spreading of epidermal cells
endodermal cell differentiation
stress fiber assembly
viral entry into host cell
epithelial cell-cell adhesion
cell-cell adhesion
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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