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Invitrogen
Phospho-Chk1 (Ser345) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-Chk1 (Ser345) Antibody (PA5-121290) in WB
Western blot analysis of Phospho-Chk1 (Ser345) in NIH/3T3 cells. Samples were incubated with Phospho-Chk1 (Ser345) Polyclonal antibody (Product # PA5-121290) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry... View More
Product Details
PA5-121290
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S345 of human Chk1 (NP_001265.2).
Conjugate
Form
Liquid
Concentration
1.27 mg/mL
Amount
127 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HT-29, HepG2, C6. The target is usually found in the following locations: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Immunogen sequence: SFSQP
Target Information
CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1; Synoviolin; unnamed protein product
Gene Aliases: C85740; CHEK1; CHK1; OZEMA21; rad27
UniProt ID: (Human) O14757, (Rat) Q91ZN7, (Mouse) O35280
Entrez Gene ID: (Human) 1111, (Rat) 140583, (Mouse) 12649
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein domain specific binding
histone kinase activity (H3-T11 specific)
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
glycosyltransferase
non-receptor serine/threonine protein kinase
DNA damage checkpoint
G2/M transition of mitotic cell cycle
inner cell mass cell proliferation
DNA replication
DNA repair
chromatin remodeling
protein phosphorylation
apoptotic process
cellular response to DNA damage stimulus
nucleus organization
nuclear envelope organization
mitotic nuclear envelope disassembly
mitotic G2 DNA damage checkpoint
regulation of gene expression
regulation of double-strand break repair via homologous recombination
positive regulation of G2/M transition of mitotic cell cycle
peptidyl-threonine phosphorylation
regulation of cell proliferation
signal transduction in response to DNA damage
mitotic G2/M transition checkpoint
positive regulation of cell cycle
negative regulation of gene expression, epigenetic
negative regulation of mitotic nuclear division
regulation of mitotic centrosome separation
negative regulation of G0 to G1 transition
cellular response to mechanical stimulus
replicative senescence
regulation of signal transduction by p53 class mediator
negative regulation of cell cycle phase transition
apoptotic process involved in development
DNA damage induced protein phosphorylation
regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage
negative regulation of G2/M transition of mitotic cell cycle
histone H3-T11 phosphorylation
chromatin-mediated maintenance of transcription
cellular response to organic substance
cellular response to caffeine
negative regulation of DNA biosynthetic process
regulation of histone H3-K9 acetylation
cell cycle
phosphorylation
G2 DNA damage checkpoint
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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