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Invitrogen

Phospho-Actin (Tyr53) Polyclonal Antibody

View all (21) Actin antibodies

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Product Details

PA5-143643

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:50
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human beta actin. This sequence is identical to similar regions in all four alpha actins, as well as in gamma actin, and is well conserved in actins from most eukaryotic species.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Amount

25 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942871

Product Specific Information

This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ACT2; ACTA1; ACTA2; ACTC; ACTG2; actin 2; actin 7; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; AtACT7; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; cytoskeletal gamma-actin; DEFORMED ROOT HAIRS 1; DER1; ENHANCER OF LRX1 2; ENL2; epididymis luminal protein 176; FIZ2; FRIZZY AND KINKED SHOOTS 2; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; I(2)-actin; LIGHT STRESS-REGULATED 2; LSR2; MYH9.2; MYH9_2; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin; unnamed protein product

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Gene Aliases: 0610041G09Rik; a-SMA; AA959943; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; beta-actin; BKRNS; BNS; BRWS1; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; CSMH; DDS1; DFNA20; DFNA26; E430023M04Rik; E51; GIG46; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMDYS; SMGA; THC8; VSCM; VSCM1

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UniProt ID: (Human) P68133, (Human) P62736, (Human) P60709, (Human) P68032, (Human) P63261, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710, (Mouse) P68033, (Rat) P68035, (Mouse) P63260, (Rat) P63259, (Mouse) P63268, (Rat) P63269

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Entrez Gene ID: (Human) 58, (Human) 59, (Human) 60, (Human) 70, (Human) 71, (Human) 72, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Mouse) 11464, (Rat) 29275, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365

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Function(s)
nucleotide binding structural constituent of cytoskeleton protein binding ATP binding hydrolase activity myosin binding ADP binding protein kinase binding ATPase activity kinesin binding nitric-oxide synthase regulator activity Tat protein binding nucleosomal DNA binding identical protein binding tau protein binding nitric-oxide synthase binding structural constituent of postsynaptic actin cytoskeleton transporter regulator activity microfilament motor activity profilin binding ubiquitin protein ligase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding molecular_function actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein
Process(es)
muscle contraction positive regulation of gene expression skeletal muscle thin filament assembly skeletal muscle fiber development mesenchyme migration regulation of blood pressure response to virus vascular smooth muscle contraction positive regulation of hepatic stellate cell migration positive regulation of hepatic stellate cell contraction positive regulation of ERK1 and ERK2 cascade cellular response to transforming growth factor beta stimulus juxtaglomerular apparatus development glomerular mesangial cell development positive regulation of hepatic stellate cell activation morphogenesis of a polarized epithelium chromatin remodeling regulation of transcription from RNA polymerase II promoter cytoskeleton organization establishment or maintenance of cell polarity axonogenesis positive regulation of cell proliferation substantia nigra development regulation of mitotic metaphase/anaphase transition adherens junction assembly maintenance of blood-brain barrier regulation of apoptotic process apical protein localization positive regulation of T cell differentiation negative regulation of cell differentiation positive regulation of cell differentiation positive regulation of myoblast differentiation positive regulation of transcription, DNA-templated cell motility regulation of norepinephrine uptake positive regulation of norepinephrine uptake regulation of cell cycle regulation of G0 to G1 transition platelet aggregation protein localization to adherens junction cellular response to cytochalasin B postsynaptic actin cytoskeleton organization regulation of transepithelial transport regulation of synaptic vesicle endocytosis positive regulation of stem cell population maintenance regulation of protein localization to plasma membrane positive regulation of double-strand break repair via homologous recombination regulation of G1/S transition of mitotic cell cycle regulation of double-strand break repair positive regulation of double-strand break repair regulation of nucleotide-excision repair actin filament organization response to xenobiotic stimulus actin filament-based movement actomyosin structure organization actin-myosin filament sliding negative regulation of apoptotic process response to ethanol cardiac myofibril assembly cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction cytoplasmic actin-based contraction involved in cell motility angiogenesis positive regulation of cell migration sarcomere organization regulation of stress fiber assembly regulation of focal adhesion assembly cellular response to interferon-gamma positive regulation of wound healing tight junction assembly protein localization to bicellular tight junction response to mechanical stimulus response to extracellular stimulus response to lithium ion cell growth skeletal muscle fiber adaptation response to steroid hormone retina homeostasis ATP-dependent chromatin remodeling retina development in camera-type eye cellular response to electrical stimulus metabolic process actin-mediated cell contraction response to drug response to calcium ion biological_process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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