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Invitrogen
Phospho-p130Cas (Tyr751) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-p130Cas (Tyr751) Antibody (PA5-143733) in WB
Western blot of Phospho-p130Cas (Tyr751) in human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with p130Cas monoclonal antibody (Product # MA5-47675) (lanes 1 & 2), Phospho-p130Cas (Tyr751) polyclonal antibody (Product # PA5-143733) (lanes 3 & 4) or Phospho-p130Cas (Tyr762) polyclonal antibody (Product # ... View More
Product Details
PA5-143733
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. This rat sequence is the same as Tyr-653 in human p130 Cas and Tyr-657 in mouse p130 Cas.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier). The antibody detects a 130 kDa* protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.
Target Information
The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCA1; BCAR1, Cas family scaffold protein; BCAR1, Cas family scaffolding protein; Breast cancer anti-estrogen resistance protein 1; Cas scaffolding protein family member 1; CRK-associated substrate; Crk-associated substrate p130Cas; OTTHUMP00000174941; p130 Cas; p130cas; p130Cas (pTyr410); p130Cas (pY410); p130Cas phospho; phospho p130Cas; unnamed protein product; v-crk-associated tyrosine kinase substrate
Gene Aliases: AI385681; BCAR1; CAS; CAS1; CASS1; CRKAS; P130CAS
UniProt ID: (Human) P56945, (Mouse) Q61140, (Rat) Q63767
Entrez Gene ID: (Human) 9564, (Mouse) 12927, (Rat) 25414
regulation of cell growth
actin filament organization
cell adhesion
transmembrane receptor protein tyrosine kinase signaling pathway
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
integrin-mediated signaling pathway
insulin receptor signaling pathway
positive regulation of endothelial cell migration
cell migration
positive regulation of cell migration
cellular response to hepatocyte growth factor stimulus
regulation of apoptotic process
platelet-derived growth factor receptor signaling pathway
vascular endothelial growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
hepatocyte growth factor receptor signaling pathway
antigen receptor-mediated signaling pathway
T cell receptor signaling pathway
B cell receptor signaling pathway
cell division
cell chemotaxis
endothelin receptor signaling pathway
actin cytoskeleton organization
signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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