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Invitrogen
Dicer Polyclonal Antibody
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FIGURE: 1 / 4
Dicer Antibody (PA5-17021) in ICC/IF
Immunofluorescence analysis of DICER was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with DICER Polyclonal Antibody (Product # PA5-17021) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731... View More
Product Details
PA5-17021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the sequence of human Dicer
Conjugate
Form
Liquid
Concentration
18 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antiviral isoform of Dicer; DCR; DCR-1; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; helicase-moi; unnamed protein product; variant of Dicer1
Gene Aliases: aviD; DCR1; DICER; DICER1; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; KIAA0928; MNG1; RMSE2
UniProt ID: (Human) Q9UPY3
Entrez Gene ID: (Human) 23405
nucleotide binding
nucleic acid binding
RNA binding
double-stranded RNA binding
helicase activity
nuclease activity
endonuclease activity
endoribonuclease activity
ribonuclease III activity
deoxyribonuclease I activity
protein binding
ATP binding
hydrolase activity
endoribonuclease activity, producing 5'-phosphomonoesters
protein domain specific binding
siRNA binding
metal ion binding
regulatory RNA binding
pre-miRNA binding
negative regulation of transcription from RNA polymerase II promoter
apoptotic DNA fragmentation
RNA processing
miRNA metabolic process
negative regulation of Schwann cell proliferation
negative regulation of gene expression
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of Schwann cell differentiation
tRNA catabolic process
posttranscriptional gene silencing
nerve development
production of siRNA involved in RNA interference
gene silencing by RNA
pre-miRNA processing
positive regulation of myelination
peripheral nervous system myelin formation
negative regulation of tumor necrosis factor production
production of miRNAs involved in gene silencing by miRNA
neuron projection morphogenesis
regulation of multicellular organismal process
small RNA loading onto RISC
global gene silencing by mRNA cleavage
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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