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Invitrogen
COX4 Polyclonal Antibody
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FIGURE: 1 / 5
COX4 Antibody (PA5-17511) in ICC/IF
Immunofluorescence analysis of COX4 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COX4 Polyclonal Antibody (Product # PA5-17511) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More
Product Details
PA5-17511
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Lys29 of human COX IV
Conjugate
Form
Liquid
Concentration
266 µg/mL
Amount
26.6 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4 (COX4I1) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cox iv; COX IV-1; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV; cytochrome c oxidase subunit IV (COX4); Cytochrome c oxidase subunit IV isoform 1; cytochrome c oxidase subunit IV precursor EC 1.9.3.1; cytochrome c oxidase, subunit 4a; cytochrome c oxidase, subunit IV; cytochrome c oxidase, subunit IVa; cytochrome c oxydase subunit 4; FLJ23483; MGC72016; unnamed protein product
Gene Aliases: AL024441; COX; COX IV-1; COX4; COX4-1; Cox4a; COX4I1; COXIV; COXIV-1; IV-1; MC4DN16
UniProt ID: (Bovine) P00423, (Human) P13073, (Rat) P10888, (Mouse) P19783
Entrez Gene ID: (Bovine) 281090, (Human) 1327, (Rat) 29445, (Mouse) 12857
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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