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Invitrogen
PPP2R4 Polyclonal Antibody
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FIGURE: 1 / 5
PPP2R4 Antibody (PA5-19136) in ICC/IF
Immunofluorescence analysis of PPP2R4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PPP2R4 Goat Polyclonal Antibody (Product # PA5-19136) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate... View More
Product Details
PA5-19136
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (GSLLPIHPVTSG) corresponding to the C-terminus amino acids of PPP2R4 (aa 347-358).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine and canine based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC2184; OTTHUMP00000022333; Phosphotyrosyl phosphatase activator; PP2A phosphatase activator; PP2A, subunit B', PR53 isoform; protein phosphatase 2 regulatory subunit 4; protein phosphatase 2A activator, regulatory subunit 4; protein phosphatase 2A regulatory subunit 4; protein phosphatase 2A, regulatory subunit B (PR 53); protein phosphatase 2A, regulatory subunit B' (PR 53); PTPA; Serine/threonine-protein phosphatase 2A activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B'; unnamed protein product
Gene Aliases: 2610042B21Rik; C77440; N28142; PARK25; PP2A; PPP2R4; PR53; PTPA
UniProt ID: (Human) Q15257, (Mouse) P58389
Entrez Gene ID: (Human) 5524, (Rat) 362102, (Mouse) 110854
nucleotide binding
peptidyl-prolyl cis-trans isomerase activity
phosphoprotein phosphatase activity
receptor binding
protein binding
ATP binding
protein tyrosine phosphatase activator activity
isomerase activity
phosphatase activator activity
protein phosphatase regulator activity
phosphatase binding
protein homodimerization activity
metal ion binding
protein phosphatase 2A binding
protein phosphatase type 2A regulator activity
ATPase activity
protein-binding activity modulator
phosphatase activator
phosphatase modulator
mitotic spindle organization
positive regulation of apoptotic process
protein peptidyl-prolyl isomerization
metabolic process
mitotic spindle organization in nucleus
negative regulation of phosphoprotein phosphatase activity
positive regulation of phosphoprotein phosphatase activity
positive regulation of protein dephosphorylation
negative regulation of protein dephosphorylation
regulation of phosphoprotein phosphatase activity
regulation of protein phosphatase type 2A activity
positive regulation of catalytic activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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