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Invitrogen
Tryptophan Hydroxylase Polyclonal Antibody
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FIGURE: 1 / 1
Tryptophan Hydroxylase Antibody (PA5-19749) in WB
Western blot analysis of Human Brain Tissue Lysate using Product # PA5-19749, Tryptophan Hydroxylase primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Mouse Brain Tissue Lysate at a dilution of 1 µg/mL (Lane 2). Staining of Serotoninergic nucleus Rat Tissue Lysate at a dilution of 1 µg/mL (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a ... View More
Product Details
PA5-19749
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Tryptophan Hydroxylase.
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Amount
100 µg
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Gel ice packs
RRID
Product Specific Information
For Western Blot, this antibody has non-specific bands at 42 kDa.
This antibody is predicted to react with rabbit, chicken, hamster, chimpanzee, gorilla and orangutan based on sequence homology.
Target Information
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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