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Invitrogen
LGR5 Polyclonal Antibody
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FIGURE: 1 / 3
LGR5 Antibody (PA5-23000) in ICC/IF
Immunocytochemistry analysis of LGR5 in HEK293 cells. Samples were incubated with LGR5 polyclonal antibody (Product # PA5-23000) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:1,000 dilution for 60 minutes. Cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Product Details
PA5-23000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an internal portion of the human GPR49/LGR5 protein (within residues 650-700).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
G protein-coupled receptor GPR49 has been reported to be expressed in brain, skeletal muscle, placenta, and spinal cord. ESTs have been isolated from normal human brain (amygdala), embryo, and placenta libraries and from human germ cell, uterus, and brain libraries. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: follicle expressed hormone; G protein-coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; leucine-rich repeat containing G protein-coupled receptor; Leucine-rich repeat-containing G-protein coupled receptor 5; orphan G protein-coupled receptor HG38; Orphan G-protein coupled receptor FEX; unnamed protein product
Gene Aliases: FEX; GPR49; GPR67; GRP49; HG38; LGR5
UniProt ID: (Human) O75473, (Mouse) Q9Z1P4
Entrez Gene ID: (Pig) 100151994, (Human) 8549, (Mouse) 14160
hair follicle development
signal transduction
G-protein coupled receptor signaling pathway
oocyte differentiation
positive regulation of Wnt signaling pathway
regulation of cell proliferation
inner ear development
positive regulation of canonical Wnt signaling pathway
epithelial cell proliferation involved in renal tubule morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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