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Invitrogen

HMOX1 Polyclonal Antibody

8 Published Figures
16 References
View all (46) HO-1 antibodies

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Product Details

PA5-27338

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 11 publications 11 publications

Immunohistochemistry (IHC)

-
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.55 mg/mL

Amount

155 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544814

Product Specific Information

Recommended positive controls: HeLa, Raw264.7, C2C12, rat spleen, HeLa (500 µM CoCl2 treatment for 24 hr).

Predicted reactivity: Mouse (81%), Rat (80%), Dog (85%), Pig (82%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HMOX1; HO-1; HSP32; P32 protein; unnamed protein product

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Gene Aliases: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

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UniProt ID: (Human) P09601, (Rat) P06762, (Mouse) P14901

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Entrez Gene ID: (Human) 3162, (Rat) 24451, (Mouse) 15368

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Function(s)
heme oxygenase (decyclizing) activity structural molecule activity protein binding oxidoreductase activity enzyme binding heme binding identical protein binding protein homodimerization activity metal ion binding phospholipase D activity signal transducer activity oxygenase
Process(es)
angiogenesis endothelial cell proliferation wound healing involved in inflammatory response negative regulation of leukocyte migration regulation of transcription from RNA polymerase II promoter heme oxidation cellular iron ion homeostasis apoptotic process response to oxidative stress smooth muscle hyperplasia macroautophagy positive regulation of macroautophagy negative regulation of macroautophagy positive regulation of chemokine production erythrocyte homeostasis low-density lipoprotein particle clearance cellular response to heat response to nicotine intracellular signal transduction heme catabolic process heme metabolic process response to hydrogen peroxide positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of angiogenesis positive regulation of angiogenesis positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation multicellular organismal iron ion homeostasis cellular response to arsenic-containing substance cellular response to cadmium ion cellular response to cisplatin positive regulation of cell migration involved in sprouting angiogenesis negative regulation of ferroptosis negative regulation of cytokine production involved in inflammatory response negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis epithelial cell apoptotic process positive regulation of epithelial cell apoptotic process response to hypoxia phospholipid metabolic process small GTPase mediated signal transduction regulation of blood pressure cell death negative regulation of cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of muscle cell apoptotic process cellular response to nutrient negative regulation of mast cell cytokine production regulation of transcription from RNA polymerase II promoter in response to iron negative regulation of mast cell degranulation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen regulation of sequence-specific DNA binding transcription factor activity protein homooligomerization iron ion homeostasis cellular response to hypoxia oxidation-reduction process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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