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Invitrogen
alpha Actinin 2 Polyclonal Antibody
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FIGURE: 1 / 14
alpha Actinin 2 Antibody (PA5-27863) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of alpha Actinin 2 was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: alpha Actinin 2 Polyclonal Antibody (Product # PA5 27863) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-27863
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat,
Zebrafish
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.18 mg/mL
Amount
18 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, Rat heart.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (94%), Pig (99%), Chicken (98%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; F-actin cross-linking protein; unnamed protein product
Gene Aliases: 1110008F24Rik; ACTN2; CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ
UniProt ID: (Human) P35609, (Mouse) Q9JI91
Entrez Gene ID: (Human) 88, (Pig) 100157406, (Mouse) 11472, (Rat) 291245
transcription coactivator activity
actin binding
integrin binding
calcium ion binding
protein binding
phosphatidylinositol-4,5-bisphosphate binding
cytoskeletal protein binding
structural constituent of muscle
protein domain specific binding
LIM domain binding
titin binding
identical protein binding
ion channel binding
metal ion binding
actin filament binding
FATZ binding
titin Z domain binding
structural constituent of postsynaptic actin cytoskeleton
channel activator activity
ligand-dependent nuclear receptor transcription coactivator activity
thyroid hormone receptor coactivator activity
protein binding, bridging
protein homodimerization activity
protein dimerization activity
muscle contraction
microspike assembly
regulation of membrane potential
negative regulation of potassium ion transport
positive regulation of potassium ion transport
focal adhesion assembly
protein homotetramerization
negative regulation of potassium ion transmembrane transporter activity
positive regulation of potassium ion transmembrane transporter activity
regulation of nucleic acid-templated transcription
negative regulation of protein localization to cell surface
positive regulation of receptor activity
cell adhesion
actin cytoskeleton organization
regulation of apoptotic process
sarcomere organization
positive regulation of transcription, DNA-templated
anatomical structure formation involved in morphogenesis
actin filament uncapping
muscle cell development
cardiac muscle cell development
protein localization to plasma membrane
phospholipase C-activating angiotensin-activated signaling pathway
postsynaptic actin cytoskeleton organization
positive regulation of endocytic recycling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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