Invitrogen

UHRF1 Polyclonal Antibody

Name: UHRF1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-27969

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3 Published Figures

2 References

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Datasheet

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Additional Information:

UHRF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

UHRF1 Antibody (PA5-27969) in ICC/IF

Immunocytochemistry (Confocal immunofluorescence) of UHRF1 in paraformaldehyde-fixed HeLa. Samples were incubated with UHRF1 polyclonal antibody (Product # PA5-27969) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,000.

UHRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

UHRF1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

UHRF1 Antibody — Fig. 6 LASP-1 associates with epigenetic proteins UHRF1, DNMT1, G9a and Snail1 A) LASP-1 co-immunoprecipitates with UHRF1 and DNMT1. 100 mug of the nuclear extracts from MDA-Bone-Un cells stimulated with vehicle (0 min) or 500 ng /ml of CXCL12 for 15 min were incubated with control mouse monoclonal IgG (Mock) or with mouse anti-LASP-1 antibody. LASP1-bound proteins were eluted and analyzed for endogenous LASP-1 and associated proteins UHRF1 and DNMT1. Bottom panel: 15 mug of total cell lysates from MDA-Bone-Un cells that were stimulated with vehicle (0 min) or CXCL12 for 15 min. LASP-1 lysate blot was obtained after stripping and re-probing for LASP-1 after the detection of Snail1 first. The experiment was repeated twice and the representative blot was quantified and shown. NS - Non-silenced control; KD - LASP-1 knock down. The band intensities of proteins were quantified by using Image J analysis and normalized to their respective non-stimulated control (bands in -CXCL12 lane). The fold change was given above the bands to track the level of the immunoprecipitated proteins with respect to their protein levels in the lysates. B) LASP-1 co-immunoprecipitates with G9a and Snail1. 100 mug of the nuclear extracts from MDA-Bone-Un cells stimulated with vehicle (0 min and Mock) or 500 ng /ml of CXCL12 for 15 min were incubated with control mouse monoclonal IgG (Mock) or with mouse anti-LASP-1 antibody. Bound proteins were eluted and analyzed for endogenous LASP-1 and associated G9a {TM}

Product Details

PA5-27969

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

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Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human UHRF1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545445

Product Specific Information

Recommended positive controls: HeLa.

Predicted reactivity: Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Ac2-121; E3 ubiquitin-protein ligase UHRF1; FLJ21925; Hnp95 Huhrf1; hUHRF1; HuNp95; inverted CCAAT box binding protein 90; Inverted CCAAT box-binding protein of 90 kDa; Liver regeneration-related protein LRRG126; MGC138707; mUhrf1; nuclear phosphoprotein 95; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; thymus and cell proliferation associated protein; Transcription factor ICBP90; ub; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like, containing PHD and RING finger domains, 1; Ubiquitin-like-containing PHD and RING finger domains protein 1; unnamed protein product

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Gene Aliases: Ac2-121; AL022808; hNP95; hUHRF1; huNp95; ICBP90; NP95; RNF106; TDRD22; UHRF1

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UniProt ID: (Human) Q96T88, (Mouse) Q8VDF2, (Rat) Q7TPK1

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Entrez Gene ID: (Human) 29128, (Mouse) 18140, (Rat) 316129

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Function(s)

core promoter proximal region sequence-specific DNA binding nucleic acid binding DNA binding ubiquitin-protein transferase activity protein binding zinc ion binding methyl-CpG binding transferase activity histone binding identical protein binding hemi-methylated DNA-binding metal ion binding ubiquitin protein ligase activity histone H3K9me2/3 reader activity DNA damage sensor activity histone H3 ubiquitin ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity nucleosomal histone binding methylated histone binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity molecular_function ubiquitin-protein ligase

Process(es)

negative regulation of transcription from RNA polymerase II promoter double-strand break repair via homologous recombination DNA repair chromatin organization ubiquitin-dependent protein catabolic process response to stress cellular response to DNA damage stimulus protein ubiquitination heterochromatin assembly reciprocal DNA recombination regulation of gene expression, epigenetic hypermethylation of CpG island positive regulation of transcription from RNA polymerase II promoter regulation of epithelial cell proliferation positive regulation of protein metabolic process protein autoubiquitination mitotic spindle assembly transcription, DNA-templated regulation of transcription, DNA-templated cell cycle cell proliferation maintenance of DNA methylation histone monoubiquitination chromatin modification histone ubiquitination positive regulation of cellular protein metabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of methylation-dependent chromatin silencing response to peptide hormone

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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UHRF1 Polyclonal Antibody

UHRF1 Antibody (PA5-27969) in ICC/IF

Product Details

PA5-27969

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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