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Invitrogen
CARD10 Polyclonal Antibody
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FIGURE: 1 / 3
CARD10 Antibody (PA5-29261) in IHC (P)
CARD10 Polyclonal Antibody detects CARD10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CARD10 Polyclonal Antibody (Product # PA5-29261) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-29261
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human CARD10. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, H1299, Molt-4, Raji, mouse heart.
Predicted reactivity: Mouse (94%), Rat (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. As with CARMA1, the CARD domain of CARMA3 has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-Kappa-B activation. When expressed in cells, this protein binds to BCL10 and activates NF-Kappa-B Recent experiments have shown that CARMA3 is required for EGF-induced NF-Kappa-B activation and contributes to tumor growth in vivo, suggesting that CARMA3 may serve as a new therapeutic target for the treatment of EGFR-driven tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl10 binding protein and activator of NFKB; Bcl10-interacting MAGUK protein 1; Bimp1; CARD-containing MAGUK 3 protein; CARD-containing MAGUK protein 3; CARD10; CARD/MAGUK family member; Carma 3; Caspase recruitment domain-containing protein 10; unnamed protein product
Gene Aliases: AI449026; BIMP1; CARD10; CARMA3; IMD89
UniProt ID: (Human) Q9BWT7, (Mouse) P58660
Entrez Gene ID: (Human) 29775, (Mouse) 105844
activation of NF-kappaB-inducing kinase activity
regulation of apoptotic process
positive regulation of macromolecule metabolic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of immune response
protein-containing complex assembly
negative regulation of cell migration involved in sprouting angiogenesis
positive regulation of protein localization to nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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