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Invitrogen
SMYD3 Polyclonal Antibody
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FIGURE: 1 / 11
SMYD3 Antibody (PA5-31919) in ICC/IF
SMYD3 Polyclonal Antibody detects SMYD3 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: SMYD3 protein stained by SMYD3 Polyclonal Antibody (Product # PA5-31919) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-31919
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.23 mg/mL
Amount
123 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: DDDDK-tagged SMYD3-transfected 293T, 293T, A431, HeLa, HepG2, A549, SKOV3, HCT116.
Predicted reactivity: Human (99%), Mouse (93%), Rat (94%), Pig (95%), Rhesus Monkey (99%), Bovine (83%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4. It induces di- and tri-methylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; SET domain; unnamed protein product; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1
Gene Aliases: 2410008A19Rik; bA74P14.1; KMT3E; SMYD3; ZMYND1; ZNFN3A1
UniProt ID: (Human) Q9H7B4, (Mouse) Q9CWR2
Entrez Gene ID: (Human) 64754, (Mouse) 69726
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
protein binding
methyltransferase activity
zinc ion binding
transferase activity
histone methyltransferase activity
metal ion binding
histone H4 methyltransferase activity
histone H3K36 dimethyltransferase activity
histone H3K4 trimethyltransferase activity
RNA polymerase II core promoter sequence-specific DNA binding
histone-lysine N-methyltransferase activity
histone modifying enzyme
chromatin organization
nucleosome assembly
myotube cell development
methylation
establishment of protein localization
positive regulation of transcription from RNA polymerase II promoter
cellular response to dexamethasone stimulus
negative regulation of protein kinase activity
chromatin modification
positive regulation of peptidyl-serine phosphorylation
histone lysine methylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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