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          • Primary Antibodies ›
          • Neuropilin 1 (CD304) Antibodies

          Invitrogen

          Neuropilin 1 Polyclonal Antibody

          Advanced Verification
          1 Reference
          View all (52) Neuropilin 1 (CD304) antibodies

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          Cite Neuropilin 1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (8)
          • Advanced Verification (2)
          Neuropilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Neuropilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          Neuropilin 1 Antibody (PA5-47027) in IHC (P)

          Immunohistochemical analysis of Neuropilin 1 in immersion fixed paraffin-embedded sections of rat brain (hippocampus). Samples were incubated with Neuropilin 1 polyclonal antibody (Product # PA5-47027) using a dilution of 3 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific s... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Neuropilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Neuropilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Neuropilin 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Neuropilin 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Neuropilin 1 Antibody in Western Blot (WB)
          Neuropilin 1 Antibody in Western Blot (WB)
          Neuropilin 1 Antibody in Western Blot (WB)
          Neuropilin 1 Antibody in Flow Cytometry (Flow)
          Neuropilin 1 Antibody
          Neuropilin 1 Antibody
          Neuropilin 1 Polyclonal Antibody

          Product Details

          PA5-47027

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          3-15 µg/mL
          -

          Immunohistochemistry (Frozen) (IHC (F))

          3-15 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          2.5 µg per million cells
          -

          Neutralization (Neu)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Goat / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Mouse myeloma cell line NS0-derived recombinant rat Neuropilin-1 Phe22-Ala810 (Lys811Arg), Ser829-Asp854
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          0.2 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 5% trehalose

          Contains

          No Preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2608228

          Product Specific Information

          In direct ELISAs, approximately 25% cross-reactivity with recombinant human Neuropilin-1 is observed, and less than 1% cross-reactivity with recombinant human Neuropilin-2, recombinant mouse Neuropilin-2, and recombinant rat Neuropilin-2 is observed.

          Reconstitute at 0.2 mg/mL in sterile PBS.

          Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

          Target Information

          Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: A5 protein; alternatively spliced; lacks exon 16; CD304; Neuropilin; Neuropilin-1; Neuropilin-1 precursor (A5 protein); Neuropilin1; NRP 1; receptor for VEGF165 and semaphorins class 3; sNRP 1; sNRP1; soluble NRP 1; soluble NRP1; transmembrane receptor; unnamed protein product; Vascular endothelial cell growth factor 165 receptor; VEGF 165 receptor

          View more View less

          Gene Aliases: BDCA4; C530029I03; CD304; NP-1; NP1; NPN-1; Npn1; NRP; NRP1; VEGF165R

          View more View less

          UniProt ID: (Human) O14786, (Rat) Q9QWJ9, (Mouse) P97333

          View more View less

          Entrez Gene ID: (Human) 8829, (Rat) 246331, (Mouse) 18186

          View more View less

          Function(s)
          vascular endothelial growth factor-activated receptor activity GTPase activator activity protein binding heparin binding coreceptor activity semaphorin receptor activity growth factor binding protein kinase binding cytokine binding vascular endothelial growth factor binding metal ion binding oxidoreductase
          Process(es)
          angiogenesis patterning of blood vessels vasculogenesis neural crest cell migration neuron migration positive regulation of endothelial cell proliferation sprouting angiogenesis cell migration involved in sprouting angiogenesis outflow tract septum morphogenesis substrate-dependent cell migration, cell extension signal transduction integrin-mediated signaling pathway cell-cell signaling nervous system development axon guidance axonal fasciculation heart development motor neuron axon guidance response to wounding organ morphogenesis positive regulation of endothelial cell migration positive regulation of smooth muscle cell migration dendrite development facial nerve structural organization trigeminal nerve morphogenesis trigeminal nerve structural organization vestibulocochlear nerve structural organization branchiomotor neuron axon guidance gonadotrophin-releasing hormone neuronal migration to the hypothalamus cell differentiation negative regulation of axon extension regulation of vascular endothelial growth factor receptor signaling pathway retinal ganglion cell axon guidance regulation of Cdc42 protein signal transduction cellular response to hepatocyte growth factor stimulus endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus ventral trunk neural crest cell migration vascular endothelial growth factor signaling pathway neuropilin signaling pathway VEGF-activated neuropilin signaling pathway positive regulation of phosphorylation negative regulation of neuron apoptotic process blood vessel endothelial cell migration endothelial cell migration positive regulation of angiogenesis viral entry into host cell platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway hepatocyte growth factor receptor signaling pathway sympathetic nervous system development neuron development axon extension regulation of axon extension involved in axon guidance positive regulation of axon extension involved in axon guidance negative regulation of axon extension involved in axon guidance artery morphogenesis axon extension involved in axon guidance positive chemotaxis positive regulation of filopodium assembly positive regulation of stress fiber assembly positive regulation of focal adhesion assembly axonogenesis involved in innervation regulation of vesicle-mediated transport dichotomous subdivision of terminal units involved in salivary gland branching angiogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis retina vasculature development in camera-type eye retina vasculature morphogenesis in camera-type eye renal artery morphogenesis sympathetic ganglion development trigeminal ganglion development positive regulation of ERK1 and ERK2 cascade semaphorin-plexin signaling pathway commissural neuron axon guidance positive regulation of cell migration involved in sprouting angiogenesis endothelial tip cell fate specification sensory neuron axon guidance motor neuron migration sympathetic neuron projection extension sympathetic neuron projection guidance postsynapse organization regulation of plasma membrane bounded cell projection organization basal dendrite development basal dendrite arborization positive regulation of substrate adhesion-dependent cell spreading neural crest cell migration involved in autonomic nervous system development protein localization to early endosome facioacoustic ganglion development dorsal root ganglion morphogenesis otic placode development negative regulation of extrinsic apoptotic signaling pathway cell migration peptidyl-tyrosine phosphorylation nerve development otic placode formation positive regulation of peptidyl-tyrosine phosphorylation ganglion morphogenesis regulation of retinal ganglion cell axon guidance toxin transport semaphorin-plexin signaling pathway involved in neuron projection guidance semaphorin-plexin signaling pathway involved in axon guidance positive regulation of retinal ganglion cell axon guidance VEGF-activated neuropilin signaling pathway involved in axon guidance multicellular organism development cell migration involved in coronary vasculogenesis
          It has to be done as per old AB suggested Products section.

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