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Invitrogen
MAP4K3 Polyclonal Antibody
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FIGURE: 1 / 4
MAP4K3 Antibody (PA5-48695) in IHC (P)
Immunohistochemistry analysis of MAP4K3 in formalin fixed and paraffin embedded mouse heart tissue. Samples were incubated with MAP4K3 polyclonal antibody (Product # PA5-48695) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Product Details
PA5-48695
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 501-529 amino acids from the Central region of mouse Map4k3.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Predicted to react with rat based on sequence homology.
Target Information
MAP4K3 (also known as GLK) is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. MAP4K3 is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-alpha and can lead to the activation of the JNK signaling pathway. MAP4K3 can directly activate PKC-T, during TCR signaling and people with systemic lupus erythematosus show considerable enhanced MAP4K3 expression and activation of PKC-T, and the kinase IKK in T cells. MAP4K3 can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase 1 (MEKK1). MAP4K3 previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase 3 (MAP4K3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: germinal center kinase-like kinase; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; mitogen activated protein kinase kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase kinase 3; unnamed protein product
Gene Aliases: 4833416M01Rik; 4833416M07Rik; 9530052P13Rik; GLK; MAP4K3; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
UniProt ID: (Human) Q8IVH8, (Mouse) Q99JP0
Entrez Gene ID: (Human) 8491, (Mouse) 225028
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
MAP kinase kinase kinase kinase activity
kinase activity
transferase activity
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
voltage-gated ion channel
protein phosphorylation
JNK cascade
response to UV
response to tumor necrosis factor
intracellular signal transduction
MAPK cascade
activation of MAPKKK activity
regulation of mitotic cell cycle
phosphorylation
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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