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Invitrogen
Phospho-ITK (Tyr512) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-ITK (Tyr512) Antibody (PA5-64523) in IHC (P)
Immunohistochemistry analysis of Phospho-ITK (Tyr512) on paraffin-embedded human testis tissue using Phospho-ITK (Tyr512) Polyclonal Antibody (Product # PA5-64523) at dilution 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH 8.0 was used for antigen retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.
Product Details
PA5-64523
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ITK (Phospho-Tyr512)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Phospho-ITK (Tyr512) Polyclonal Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512.
Target Information
ITK is a tyrosine kinase expressed in T-cells which contains both SH2 and SH3 domains. ITK is thought to play a role in T-cell proliferation and differentiation. The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Itk (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. Itk associates with CD28 and becomes activated subsequent to CD28 ligation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: homolog of mouse T-cell itk/tsk; IL-2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; MGC126257; MGC126258; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; unnamed protein product
Gene Aliases: EMT; ITK; LPFS1; LYK; PSCTK2; Tcsk; Tlk; Tsk
UniProt ID: (Human) Q08881, (Mouse) Q03526
Entrez Gene ID: (Human) 3702, (Mouse) 16428
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein binding
ATP binding
zinc ion binding
kinase activity
transferase activity
metal ion binding
transferase activity, transferring phosphorus-containing groups
GTPase-activating protein
positive regulation of cytokine production
NK T cell differentiation
adaptive immune response
immune system process
cellular defense response
signal transduction
intracellular signal transduction
T cell activation
gamma-delta T cell activation
T cell receptor signaling pathway
B cell receptor signaling pathway
cytokine production
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
activation of phospholipase C activity
phosphorylation
peptidyl-tyrosine phosphorylation
interferon-gamma production
interleukin-4 production
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
innate immune response
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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