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Invitrogen
Pyruvate Carboxylase Polyclonal Antibody
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FIGURE: 1 / 2
Pyruvate Carboxylase Antibody (PA5-75545) in IHC (P)
Immunohistochemistry analysis of Pyruvate Carboxylase in paraffin-embedded human liver carcinoma tissue (cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with Pyruvate Carboxylase polyclonal antibody (Product # PA5-75545) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
Product Details
PA5-75545
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 400-445 of Human Pyruvate Carboxylase.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 6.4.1.1; PCB; PYC; pyruvate carboxylase; Pyruvate carboxylase, mitochondrial; pyruvate decarboxylase; Pyruvic carboxylase; unnamed protein product
Gene Aliases: PC; PCB; Pcx
UniProt ID: (Human) P11498, (Rat) P52873
Entrez Gene ID: (Human) 5091, (Rat) 25104, (Mouse) 18563
gluconeogenesis
lipid metabolic process
NADP metabolic process
negative regulation of gene expression
viral RNA genome packaging
viral release from host cell
NAD metabolic process
small molecule metabolic process
positive regulation by host of viral process
pyruvate metabolic process
oxaloacetate metabolic process
positive regulation by host of viral release from host cell
metabolic process
positive regulation of phospholipid biosynthetic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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