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Invitrogen
IRGM Polyclonal Antibody
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FIGURE: 1 / 2
IRGM Antibody (PA5-77775) in ICC/IF
Immunofluorescent analysis of IRGM in human colon carcinoma cell line (RKO). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with IRGM polyclonal antibody (Product # PA5-77775) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Rabbit 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: A) DAPI nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) IRGM Antibody, D) Merged image. Magnification: 60x.
Product Details
PA5-77775
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from the N-terminal of Human IRGM
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
Product Specific Information
A 1:1,000 dilution of PA5-77775 was sufficient for detection of IRGM in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 20 kDa.
Target Information
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. In mouse, IRGM belongs to a family of -gamma-interferon-induced GTP-binding proteins of approximately 48 kDa. Murine IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. Human IRGM is also involved in autophagy and plays a role in the control of intracellular pathogens and in the reduction of intracellular bacillary load.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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