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Invitrogen
DGCR8 Polyclonal Antibody
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FIGURE: 1 / 4
DGCR8 Antibody (PA5-79151) in ICC/IF
Immunocytochemistry analysis of DGCR8 using anti-DGCR8 antibody (Product # PA5-79151) . DGCR8 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DGCR8 antibody (Product # PA5-79151) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. V... View More
Product Details
PA5-79151
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human DGCR8 recombinant protein (Position: K561-Q762).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human placenta tissue, human COLO-320 whole cell, human HepG2 whole cell, human A549 whole cell, human MCF-7 whole cell, human 22RV1 whole cell. IHC: human rectal cancer tissue, mouse small intestine tissue. ICC/IF: A431 cell.
Target Information
This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DiGeorge syndrome critical region 8; DiGeorge syndrome critical region 8 homolog; DiGeorge syndrome critical region gene 8; Em:AC006547.4; Gy1; Microprocessor complex subunit DGCR8; unnamed protein product
Gene Aliases: C22orf12; D16H22S1742E; D16H22S788E; D16Wis2; DGCR8; DGCRK6; Gy1; LP4941; mir-1306; N41; pasha; Vo59c07
UniProt ID: (Human) Q8WYQ5, (Mouse) Q9EQM6
Entrez Gene ID: (Human) 54487, (Mouse) 94223, (Rat) 287954
RNA binding
double-stranded RNA binding
protein binding
heme binding
protein binding, bridging
identical protein binding
protein homodimerization activity
metal ion binding
primary miRNA binding
protein-RNA adaptor activity
ribonuclease III activity
molecular_function
RNA processing factor
scaffold/adaptor protein
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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