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Invitrogen
HLA-C Polyclonal Antibody
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FIGURE: 1 / 6
HLA-C Antibody (PA5-79367) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of HLA-C in HELA Cell using HLA-C Polyclonal Antibody (Product # PA5-79367). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 1 µg/mL. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Product Details
PA5-79367
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C (82-106aa EYWDRETQKYKRQAQADRVNLRKLR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A431 whole cell, human 293T whole cell, human Jurkat whole cell, human U20S whole cell, human Hela whole cell, human HepG2 whole cell. IHC: Human Lung Cancer tissue. ICC/IF: HELA cell. Flow: SiHa cell.
Target Information
HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: exons 2-8; HLA class I histocompatibility antigen, C alpha chain; HLA-C; HLA-C antigen; HLA-Cw; HLA-Cw1 protein; HLA-Cw2 protein; HLA-Cw3 protein; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; major histocompatibility antigen HLA-C; major histocompatibility HLA-Cw2.2 heavy chain; MHC class I antigen heavy chain HLA-C; MHC HLA-C-alpha-2 chain precursor; unnamed protein product
Gene Aliases: D6S204; HLA-C; HLA-JY3; HLAC; HLC-C; MHC; PSORS1
UniProt ID: (Human) P10321
Entrez Gene ID: (Human) 3107
positive regulation of T cell mediated cytotoxicity
adaptive immune response
immune system process
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of endogenous peptide antigen via MHC class Ib
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent
immune response
signal transduction
antigen processing and presentation
innate immune response
positive regulation of natural killer cell mediated cytotoxicity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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