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Invitrogen
MNAT1 Polyclonal Antibody
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FIGURE: 1 / 7
MNAT1 Antibody (PA5-79690) in ICC/IF
Immunocytochemistry analysis of MNAT1 using anti-MNAT1 antibody (Product # PA5-79690) . MNAT1 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-MNAT1 antibody (Product # PA5-79690) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. V... View More
Product Details
PA5-79690
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MNAT1 recombinant protein (Position: L92-A278).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human A431 whole cell, human A375 whole cell, human MCF-7 whole cell, rat testis tissue, rat L6 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell. IHC: mouse testis tissue, rat testis tissue, human testis tissue. ICC/IF: A431 cell. Flow: A431 cell.
Target Information
Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires phosphorylation at Thr 160. The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit, a regulatory subunit and a subunit of unknown function. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. This previously undescribed protein, now termed Mat1, has been cloned as a protein that associates with the cyclin H-Cdk7 nuclear complex at all stages of the cell cycle. Cyclin H-Cdk7-Mat1 complexes display kinase activity towards Cdk activation domains, and the carboxy terminus of RNA polymerase II. Mat1 appears to constitute the first example of an assembly factor, essential for the formation of an active Cdk-cyclin complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1500GX1; AdoMet synthetase; AMS1; CDK-activating kinase assembly factor MAT1; CDK7/cyclin H assembly factor; CDK7/cyclin-H assembly factor; cyclin G1 interacting protein; Cyclin-G1-interacting protein; EC 2.5.1.6; MAT-I/III; MATA1; Menage a trois; menage a trois 1; menage a trois 1 (CAK assembly factor); menage a trois homolog 1 cyclin H assembly factor (Xenopus laevis) isoform MNAT1_1; menage a trois homolog 1 cyclin H assembly factor (Xenopus laevis) isoform MNAT1_2; menage a trois homolog 1, cyclin H assembly factor; menage a trois-like protein 1 cyclin H assembly factor; MNAT CDK-activating kinase assembly factor 1; MNAT1; MNAT1, CDK activating kinase assembly factor; p35; p36; RING finger protein 66; RING finger protein MAT1
Gene Aliases: CAP35; E130115E11Rik; MAT1; MNAT1; P36; RNF66; TFB3
UniProt ID: (Human) P51948, (Mouse) P51949
Entrez Gene ID: (Human) 4331, (Mouse) 17420, (Rat) 266713
G1/S transition of mitotic cell cycle
DNA repair
nucleotide-excision repair
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
adult heart development
ventricular system development
negative regulation of apoptotic process
positive regulation of smooth muscle cell proliferation
response to calcium ion
regulation of G1/S transition of mitotic cell cycle
regulation of cyclin-dependent protein serine/threonine kinase activity
transcription, DNA-templated
transcription from RNA polymerase II promoter
protein phosphorylation
cell cycle
positive regulation of transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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