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Invitrogen
BARD1 Polyclonal Antibody
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FIGURE: 1 / 5
BARD1 Antibody (PA5-85707) in ICC/IF
BARD1 Polyclonal Antibody detects BARD1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BARD1 protein stained by BARD1 Polyclonal Antibody (Product # PA5-85707) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-85707
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human BARD1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.58 mg/mL
Amount
58 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Positive Control: A431, A431 nuclear extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BARD 1; BARD-1; BRCA1-associated RING domain gene 1; BRCA1-associated RING domain protein 1; RING-type E3 ubiquitin transferase BARD1; unnamed protein product
Gene Aliases: BARD1; ENSMUSG00000060893; ENSMUSG00000073653
UniProt ID: (Human) Q99728, (Mouse) O70445
Entrez Gene ID: (Human) 580, (Mouse) 12021
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
kinase binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
ubiquitin protein ligase activity
ubiquitinated histone binding
histone H2AK127 ubiquitin ligase activity
histone H2AK129 ubiquitin ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
tissue homeostasis
DNA repair
regulation of DNA repair
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
protein ubiquitination
negative regulation of mRNA 3'-end processing
reciprocal DNA recombination
regulation of phosphorylation
positive regulation of apoptotic process
negative regulation of apoptotic process
mitotic G2/M transition checkpoint
positive regulation of protein catabolic process
negative regulation of cell cycle
negative regulation of protein export from nucleus
regulation of cell cycle
cellular response to ionizing radiation
protein K6-linked ubiquitination
DNA strand resection involved in replication fork processing
regulation of DNA damage checkpoint
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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