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Invitrogen
Phospho-SHC (Tyr239, Tyr240) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-SHC (Tyr239, Tyr240) Antibody (PA5-85826) in WB
Western Blot using Phospho-SHC (Tyr239, Tyr240) Polyclonal Antibody (Product # PA5-85826). Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Phospho-SHC (Tyr239, Tyr240) Polyclonal Antibody (Product # PA5-85826) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal... View More
Product Details
PA5-85826
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr239/Tyr240 of human SHC1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.83 mg/mL
Amount
183 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%).
Positive Control: HepG2 (starvation for 16 hr and 200 ng/ml EGF treatment for 30 min).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ26504; OTTHUMP00000035472; RP11-307C12.1; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; unnamed protein product
Gene Aliases: SHC; SHC1; SHCA
UniProt ID: (Human) P29353
Entrez Gene ID: (Human) 6464
phosphotyrosine binding
transmembrane receptor protein tyrosine kinase adaptor activity
epidermal growth factor receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
phospholipid binding
receptor tyrosine kinase binding
ephrin receptor binding
epidermal growth factor binding
angiogenesis
epidermal growth factor receptor signaling pathway
heart development
positive regulation of cell proliferation
insulin receptor signaling pathway
negative regulation of angiogenesis
actin cytoskeleton organization
intracellular signal transduction
regulation of cell proliferation
defense response to bacterium
negative regulation of apoptotic process
positive regulation of MAPK cascade
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
insulin-like growth factor receptor signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to growth factor stimulus
positive regulation of cell proliferation in bone marrow
regulation of superoxide metabolic process
cell-cell adhesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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