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Invitrogen
PDCD4 Polyclonal Antibody
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FIGURE: 1 / 5
PDCD4 Antibody (PA5-86355) in ICC/IF
Immunofluorescence analysis of Programmed cell death protein 4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PDCD4 Polyclonal Antibody (Product # PA5-86355) at 1:100 dilution n 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Al... View More
Product Details
PA5-86355
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 421-469 of Human PDCD4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Death up-regulated gene protein; death-upregulated; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; RP11-348N5.4; Topoisomerase-inhibitor suppressed protein; unnamed protein product
Gene Aliases: D19Ucla1; Dug; H731; Ma3; PDCD4; Tis
UniProt ID: (Human) Q53EL6, (Rat) Q9JID1, (Mouse) Q61823
Entrez Gene ID: (Human) 27250, (Rat) 64031, (Mouse) 18569
apoptotic process
BMP signaling pathway
negative regulation of JUN kinase activity
negative regulation of transcription, DNA-templated
positive regulation of inflammatory response
epithelial to mesenchymal transition involved in cardiac fibroblast development
cellular response to lipopolysaccharide
negative regulation of cytokine production involved in inflammatory response
positive regulation of NIK/NF-kappaB signaling
negative regulation of vascular smooth muscle cell proliferation
negative regulation of myofibroblast differentiation
negative regulation of vascular smooth muscle cell differentiation
positive regulation of vascular associated smooth muscle cell apoptotic process
positive regulation of endothelial cell apoptotic process
cell aging
positive regulation of smooth muscle cell apoptotic process
negative regulation of apoptotic process
regulation of protein metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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