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Invitrogen
CHD9 Polyclonal Antibody
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FIGURE: 1 / 1
CHD9 Antibody (PA5-88379) in WB
Western blot analysis of CHD9 in extracts of various cell lines. Samples were incubated with CHD9 Polyclonal antibody (Product # PA5-88379) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-88379
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 85-190 of human CHD9 (NP_0794104)
Conjugate
Form
Liquid
Concentration
1.27 mg/mL
Amount
127 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SFGSPAEHVL SPHSQFNCSP IHPQNQPNGL FPDVSDGSPM WGHQTATTIS NQNGSPFHQQ GHSHSMHQNK SFVAHHDFAL FQANEQQTQC TSLRSQQNRN NLNPGQ; Positive Samples: U-87MG, HeLa, Jurkat, A-549, 293t, K-562, Mouse kidney; Cellular Location: Cytoplasm, Nucleus
Target Information
CHD9 (chromodomain-helicase-DNA-binding protein 9), also known as chromatin-related mesenchymal modulator (CReMM), PPAR-α-interacting complex protein, kismet homolog 2 or CHROM1, is a 2,897 amino acid protein belonging to the Snf2/Rad54 helicase family. The CHD family of proteins are ATP-dependent chromatin remodeling enzymes which combine chromodomains with SWI2/Snf2 ATPase/helicase motifs and DNA-binding capability. Localized to the cytoplasm and the nucleus, CHD9 contains two chromodomains, one ATPbinding helicase domain and one C-terminal helicase domain. Chromodomains are protein regions of about 40-50 amino acid residues found in proteins associated with chromatin remodeling and manipulation. The domain is highly conserved among both plants and animals and is found in a large variety of proteins from many genomes. CHD9 acts as a transcriptional coactivator for PPARα and may also be an ATP-dependent chromatin remodeling protein. CHD9 is widely expressed at low levels and is present as three isoforms produced by alternative splicing.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alternatively spliced; ATP-dependent chromatin remodeler CHD9; ATP-dependent helicase CHD9; BC022889; CHD-9; chromatin remodeling factor CHROM1; Chromatin-related mesenchymal modulator; Chromatin-remodeling factor CHROM1; Chromo domain-containing protein 9; chromodomain helicase; chromodomain-helicase-DNA-binding protein 9; ciprofibrate bound protein p240; ciprofibrate-bound protein PRIC320; CReMM; DNA-helicase; FLJ12178; helicase chromatin remodeling factor; KIAA0308; Kismet homolog 2; Peroxisomal proliferator-activated receptor A-interacting complex 320 kDa protein; PPAR-alpha-interacting complex protein 320 kDa; PPAR{gamma}-interacting cofactor 320 kDa; proteinx0008; unnamed protein product
Gene Aliases: 1810014J18Rik; 9030205D12Rik; A330063D19Rik; AD-013; AD013; CHD-9; CHD9; CReMM; KIAA0308; KISH2; mKIAA0308; PRIC320; x0008
UniProt ID: (Human) Q3L8U1, (Mouse) Q8BYH8
Entrez Gene ID: (Human) 80205, (Mouse) 109151
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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