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Invitrogen
GPX1 Polyclonal Antibody
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FIGURE: 1 / 3
GPX1 Antibody (PA5-95206) in IHC (P)
Immunohistochemistry (Paraffin) analysis of GPX1 in paraffin-embedded section of human colorectal adenocarcinoma tissue using GPX1 Polyclonal Antibody (Product # PA5-95206). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution... View More
Product Details
PA5-95206
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GPX1 (116-146aa EVNGAGAHPLFAFLREALPAPSDDATALMTD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human THP-1 whole cell, rat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue. IHC: human colorectal adenocarcinoma tissue, rat lung tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cellular glutathione peroxidase; Glutathione peroxidase 1; GPx-1; GSHPx-1; HGNC:4553; MGC14399; MGC88245; OTTHUMP00000210766; Phospholipid-hydroperoxide glutathione peroxidase GPX1; Selenium-dependent glutathione peroxidase 1; selenoprotein GPX1; unnamed protein product
Gene Aliases: AI195024; AL033363; CGPx; Gpx; GPx-1; GPX1; GPXD; GSHPx; GSHPx-1; GSHPX1
UniProt ID: (Human) P07203, (Rat) P04041, (Mouse) P11352
Entrez Gene ID: (Human) 2876, (Rat) 24404, (Mouse) 14775
response to reactive oxygen species
temperature homeostasis
endothelial cell development
negative regulation of inflammatory response to antigenic stimulus
lipid metabolic process
triglyceride metabolic process
glutathione metabolic process
apoptotic process
response to oxidative stress
sensory perception of sound
intrinsic apoptotic signaling pathway in response to oxidative stress
response to xenobiotic stimulus
response to symbiotic bacterium
response to wounding
response to toxic substance
UV protection
response to hormone
response to glucose
response to selenium ion
response to gamma radiation
myotube differentiation
arachidonic acid metabolic process
lipoxygenase pathway
response to estradiol
response to lipopolysaccharide
response to hydroperoxide
response to vitamin E
regulation of mammary gland epithelial cell proliferation
cellular response to oxidative stress
response to nicotine
regulation of gene expression, epigenetic
vasodilation
response to hydrogen peroxide
hydrogen peroxide catabolic process
skeletal muscle tissue regeneration
blood vessel endothelial cell migration
fat cell differentiation
myoblast differentiation
cell redox homeostasis
fibroblast proliferation
skeletal muscle fiber development
neuron apoptotic process
myoblast proliferation
response to folic acid
interaction with symbiont
positive regulation of protein kinase B signaling
heart contraction
angiogenesis involved in wound healing
regulation of proteasomal protein catabolic process
cellular response to glucose stimulus
negative regulation of release of cytochrome c from mitochondria
cellular oxidant detoxification
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of supramolecular fiber organization
response to lipid hydroperoxide
aging
cell proliferation
response to organic cyclic compound
protein oxidation
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of neuron apoptotic process
oxidation-reduction process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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