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Invitrogen
Cytokeratin 18 Polyclonal Antibody
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FIGURE: 1 / 22
Cytokeratin 18 Antibody (PA5-95416) in IHC (P)
Immunohistochemistry (Paraffin) analysis of Cytokeratin 18 in paraffin-embedded section of human intestinal cancer tissues using Cytokeratin 18 Polyclonal Antibody (Product # PA5-95416). Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary anti... View More
Product Details
PA5-95416
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human CACO-2 whole cell, human A549 whole cell, human A431 whole cell, rat liver tissue, rat thymus tissue, mouse liver tissue, mouse thymus tissue. IHC: mouse intestine tissue, mouse kidney tissue, rat intestine tissue, rat kidney tissue, human intestinal cancer tissue, human placenta tissue, human colon cancer tissue, human lung cancer tissue, human ovarian cancer tissue, human pancreas cancer tissue, human liver cancer tissue, human stomach cancer tissue. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; Endo B; K 18; K18; keratin 18, type I; keratin complex 1, acidic, gene 18; Keratin D; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; krt18 {ECO:0000250|UniProtKB:P05783}; ORF; PIG46; type I cytoskeletal 18; unnamed protein product
Gene Aliases: CK-18; CK18; CYK18; K18; Kerd; Krt1-18; KRT18; PIG46
UniProt ID: (Human) P05783, (Rat) Q5BJY9, (Mouse) P05784
Entrez Gene ID: (Human) 3875, (Rat) 294853, (Mouse) 16668
biological_process
tumor necrosis factor-mediated signaling pathway
Golgi to plasma membrane CFTR protein transport
negative regulation of apoptotic process
intermediate filament cytoskeleton organization
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
anatomical structure morphogenesis
Golgi to plasma membrane protein transport
cell-cell adhesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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