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Invitrogen
MNAT1 Polyclonal Antibody
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FIGURE: 1 / 2
MNAT1 Antibody (PA5-95988) in WB
Western blot analysis of MNAT1 in A-431 cells. Samples were incubated with MNAT1 Polyclonal antibody (Product # PA5-95988) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-95988
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MNAT1 (NP_0024221)
Conjugate
Form
Liquid
Concentration
2.63 mg/mL
Amount
263 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE EVEEIVFNLT NNVDLDNTKK KMEIYQKENK DVIQKNKLKL TREQEELEEA LEVERQENEQ RRLFIQKEEQ LQQILKRKNK QAFLDELESS DLPVALLLAQ HKDRSTQLEM QLEKPKPVKP VTFSTGIKMG QHISLAPIHK LEEALYEYQP LQIETYGPHV PELEMLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA FSGLFWQPS; Positive Samples: PC-3, 22Rv1, DU 145, 293T, Mouse testis, Mouse liver; Cellular Location: Nucleus
Target Information
Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires phosphorylation at Thr 160. The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit, a regulatory subunit and a subunit of unknown function. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. This previously undescribed protein, now termed Mat1, has been cloned as a protein that associates with the cyclin H-Cdk7 nuclear complex at all stages of the cell cycle. Cyclin H-Cdk7-Mat1 complexes display kinase activity towards Cdk activation domains, and the carboxy terminus of RNA polymerase II. Mat1 appears to constitute the first example of an assembly factor, essential for the formation of an active Cdk-cyclin complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1500GX1; AdoMet synthetase; AMS1; CDK-activating kinase assembly factor MAT1; CDK7/cyclin H assembly factor; CDK7/cyclin-H assembly factor; cyclin G1 interacting protein; Cyclin-G1-interacting protein; EC 2.5.1.6; MAT-I/III; MATA1; Menage a trois; menage a trois 1 (CAK assembly factor); menage a trois homolog 1 cyclin H assembly factor (Xenopus laevis) isoform MNAT1_1; menage a trois homolog 1 cyclin H assembly factor (Xenopus laevis) isoform MNAT1_2; menage a trois homolog 1, cyclin H assembly factor; menage a trois-like protein 1 cyclin H assembly factor; MNAT CDK-activating kinase assembly factor 1; MNAT1; MNAT1, CDK activating kinase assembly factor; p35; p36; RING finger protein 66; RING finger protein MAT1
Gene Aliases: CAP35; E130115E11Rik; MAT1; MNAT1; P36; RNF66; TFB3
UniProt ID: (Human) P51948, (Mouse) P51949
Entrez Gene ID: (Human) 4331, (Mouse) 17420
G1/S transition of mitotic cell cycle
DNA repair
nucleotide-excision repair
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
adult heart development
ventricular system development
negative regulation of apoptotic process
positive regulation of smooth muscle cell proliferation
response to calcium ion
regulation of G1/S transition of mitotic cell cycle
regulation of cyclin-dependent protein serine/threonine kinase activity
transcription, DNA-templated
transcription from RNA polymerase II promoter
protein phosphorylation
cell cycle
positive regulation of transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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