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Invitrogen
CD62E (E-selectin) Polyclonal Antibody
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FIGURE: 1 / 1
CD62E (E-selectin) Antibody (PA5-96091) in WB
Western blot analysis of CD62E (E-selectin) in SK-BR-3 cells. Samples were incubated with CD62E (E-selectin) Polyclonal antibody (Product # PA5-96091) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-96091
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
1.93 mg/mL
Amount
193 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: WSYNTSTEAM TYDEASAYCQ QRYTHLVAIQ NKEEIEYLNS ILSYSPSYYW IGIRKVNNVW VWVGTQKPLT EEAKNWAPGE PNNRQKDEDC VEIYIKREKD VGMWNDERCS KKKLALCYTA ACTNTSCSGH GECVETINNY TCKCDPGFSG LKCEQIVNCT ALESPEHGSL VCSHPLGNFS YNSSCSISCD RGYLPSSMET MQCMSSGEW; Positive Samples: MCF7, HepG2, HeLa, Raji, Mouse liver; Cellular Location: Cell membrane, Single-pass type I membrane protein
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; selectin, endothelial cell; unnamed protein product
Gene Aliases: CD62E; E-selectin; ELAM; Elam-1; ELAM1; ESEL; LECAM2; SELE; selectin-e
UniProt ID: (Human) P16581, (Rat) P98105, (Mouse) Q00690
Entrez Gene ID: (Human) 6401, (Rat) 25544, (Mouse) 20339
positive regulation of receptor internalization
leukocyte migration involved in inflammatory response
positive regulation of leukocyte migration
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
phospholipase C-activating G-protein coupled receptor signaling pathway
calcium-mediated signaling
actin filament-based process
response to lipopolysaccharide
response to cytokine
response to tumor necrosis factor
regulation of inflammatory response
leukocyte migration
leukocyte tethering or rolling
response to interleukin-1
cell-cell adhesion via plasma-membrane adhesion molecules
positive regulation of leukocyte tethering or rolling
activation of phospholipase C activity
signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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