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Invitrogen
Securin Polyclonal Antibody
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FIGURE: 1 / 4
Securin Antibody (PA5-96997) in IHC (P)
Immunohistochemistry analysis of Securin in paraffin-embedded human lung cancer. Samples were incubated with Securin Polyclonal antibody (Product # PA5-96997) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-96997
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human PTTG1 (NP_0012693111)
Conjugate
Form
Liquid
Concentration
0.87 mg/mL
Amount
87 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI; Positive Samples: SW480, Jurkat, HeLa, Mouse stomach, Mouse thymus, Mouse spleen, Rat testis; Cellular Location: Cytoplasm, Nucleus
Target Information
The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: endogenous cardiac regeneration-associated regulator; Esp1-associated protein; ESP1-associated protein 1; HTTG; oncoprotein; MGC126883; MGC138276; Pituitary tumor transforming; pituitary tumor-transforming 1; Pituitary tumor-transforming gene 1 protein; PTTG1; Securin; Tumor-transforming protein 1; unnamed protein product
Gene Aliases: AW555095; C87862; EAP1; ECRAR; HPTTG; PTTG; PTTG1; Pttg3; TUTR1
UniProt ID: (Human) O95997, (Mouse) Q9CQJ7, (Rat) P97613
Entrez Gene ID: (Human) 9232, (Mouse) 30939, (Rat) 64193
DNA repair
cellular response to DNA damage stimulus
chromosome segregation
spermatogenesis
homologous chromosome segregation
chromosome organization
cell division
regulation of cell growth
cell cycle
mitotic nuclear division
negative regulation of cell proliferation
cellular process
negative regulation of endopeptidase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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