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Invitrogen
DARPP-32 Polyclonal Antibody
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FIGURE: 1 / 2
DARPP-32 Antibody (PA5-17852) in WB
Western blot was performed using DARPP-32 Polyclonal Antibody (Product # PA5-17852) and a ~30 kDa band corresponding to PPP1R1B was observed in Mouse Brain and Rat Brain; and was absent in Mouse Ovary and Rat Ovary. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Ovary (Lane 2), Rat Brain (Lane 3) and Rat Ovary (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product... View More
Product Details
PA5-17852
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Thr34 of human DARPP-32
Conjugate
Form
Liquid
Concentration
35.7 µg/mL
Amount
3.57 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DARPP-32; dopamine and cAMP-regulated neuronal phosphoprotein 32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; PKA-potentiated phosphatase 1 inhibitory protein PP1; PPR1B; protein pho; Protein phosphatase 1 regulatory subunit 1B; unnamed protein product
Gene Aliases: AU040756; DARPP-32; DARPP32; PPP1R1B
UniProt ID: (Human) Q9UD71, (Rat) Q6J4I0, (Mouse) Q60829
Entrez Gene ID: (Human) 84152, (Rat) 360616, (Mouse) 19049
protein kinase inhibitor activity
cAMP-dependent protein kinase inhibitor activity
protein phosphatase inhibitor activity
protein binding
protein phosphatase regulator activity
protein serine/threonine phosphatase inhibitor activity
D1 dopamine receptor binding
D2 dopamine receptor binding
D3 dopamine receptor binding
D4 dopamine receptor binding
D5 dopamine receptor binding
phosphatase inhibitor
response to amphetamine
transcription, DNA-templated
signal transduction
negative regulation of female receptivity
locomotory behavior
visual learning
intracellular signal transduction
response to morphine
behavioral response to cocaine
cellular response to cocaine
negative regulation of protein kinase activity
negative regulation of catalytic activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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