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Invitrogen
MCL-1 Polyclonal Antibody
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FIGURE: 1 / 1
MCL-1 Antibody (PA5-81443) in WB
Western Blot using MCL-1 Polyclonal Antibody (Product # PA5-81443) at 1:500 dilution. Lane A: Mouse lung tissue lysate. Lysates/proteins at 30 μg per lane Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 33 kDa.
Product Details
PA5-81443
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Mouse Mcl-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Mouse Mcl-1.
Target Information
MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl-2-related protein EAT/mcl1; Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; MCL-1S; MGC104264; MGC1839
Gene Aliases: AW556805; Mcl-1; Mcl1
UniProt ID: (Mouse) P97287
Entrez Gene ID: (Mouse) 17210
apoptotic process
multicellular organism development
intrinsic apoptotic signaling pathway in response to DNA damage
apoptotic mitochondrial changes
cell differentiation
response to cytokine
regulation of apoptotic process
negative regulation of apoptotic process
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
negative regulation of anoikis
regulation of response to DNA damage stimulus
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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