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PACRG Antibody (600-401-474) in WB
Western blot using Rocklands Affinity Purified anti-PACRG antibody shows detection of a band ~40 kDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 µg of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes ... View More
Product Details
600-401-474
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human PACRG protein.
Conjugate
Form
Liquid
Concentration
1.6 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human PACRG protein. A BLAST analysis was used to suggest reactivity with this protein from human, mouse and chicken based on 100% homology for the immunogen sequence. Cross reactivity with PACRG protein from zebrafish and rat may occur as this sequence only varies by one amino acid residue (94% homology). Expect reactivity with both splice variants of this protein. Cross reactivity with PACRG homologues from other sources has not been determined.
Target Information
PACRG is a protein that is conserved across metazoans. In vertebrates, this gene is linked in a head-to-head arrangement with the adjacent parkin gene, which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones, including heat shock proteins 70 and 90, and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients, and it suppresses unfolded Pael receptor-induced neuronal cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ32724; Hypertension-related protein 1-like protein; Molecular chaperone/chaperonin-binding protein; PARK2 co-regulated; PARK2 coregulated gene protein; parkin co-regulated gene protein; parkin coregulated; Parkin coregulated gene protein; Parkin coregulated gene protein homolog; RP3-495O10.2
Gene Aliases: 1700008H23Rik; GLUP; HAK005771; PACRG; PARK2CRG
UniProt ID: (Human) Q96M98, (Mouse) Q9DAK2
Entrez Gene ID: (Chicken) 421576, (Human) 135138, (Mouse) 69310
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