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PACSIN1 Antibody (PA5-90737) in WB
Western blot analysis of PACSIN1 in extracts of U-251MG cells. Samples were incubated with PACSIN1 Polyclonal antibody (Product # PA5-90737) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-90737
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 240-390 of human PACSIN1 (NP_0658551)
Conjugate
Form
Liquid
Concentration
2.08 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806213
Product Specific Information
Immunogen sequence: KRLVFLKEVL LDIKRHLNLA ENSSYIHVYR ELEQAIRGAD AQEDLRWFRS TSGPGMPMNW PQFEEWNPDL PHTTTKKEKQ PKKAEGVALT NATGAVESTS QAGDRGSVSS YDRGQPYATE WSDDESGNPF GGSETNGGAN PFEDDSKGVR V; Positive Samples: U-251MG; Cellular Location: Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle membrane, Membrane, Peripheral membrane protein, cytosol, ruffle membrane, synapse, synaptosome
Target Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein kinase C and casein kinase substrate in neurons protein 1; syndapin 1; syndapin I; Syndapin-1
Gene Aliases: KIAA1379; PACSIN1; SDPI
UniProt ID: (Human) Q9BY11
Entrez Gene ID: (Human) 29993
actin filament organization
regulation of endocytosis
negative regulation of endocytosis
synaptic vesicle endocytosis
neuron projection morphogenesis
protein localization to membrane
establishment of protein localization to plasma membrane
membrane tubulation
positive regulation of dendrite development
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