Proteintech

PAFAH Polyclonal Antibody

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PAFAH Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed RAW 264.7 cells using 15526-1-AP (PAFAH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PAFAH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 15526-1-AP (PAFAH antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PAFAH Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15526-1-AP (PAFAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

15526-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PAFAH Fusion Protein Ag7227 (92-438 aa encoded by BC038452) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: RLDTLWIPN KEYFWGLSKF LGTHWLMGNI LRLLFGSMTT PANWNSPLRP GEKYPLVVFS HGLGAFRTLY SAIGIDLASH GFIVAAVEHR DRSASATYYF KDQSAAEIGD KSWLYLRTLK QEEETHIRNE QVRQRAKECS QALSLILDID HGKPVKNALD LKFDMEQLKD SIDREKIAVI GHSFGGATVI QTLSEDQRFR CGIALDAWMF PLGDEVYSRI PQPLFFINSE YFQYPANIIK MKKCYSPDKE RKMITIRGSV HQNFADFTFA TGKIIGHMLK LKGDIDSNAA IDLSNKASLA FLQKHLGLHK DFDQWDCLIE GDDENLIPGT NINTTNQHIM LQNSSGIE (92-438 aa encoded by BC038452 )

Target Information

Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL-associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

VDAC2 enables BAX to mediate apoptosis and limit tumor development.

Nature communications

Chin HS,Li MX,Tan IKL,Ninnis RL,Reljic B,Scicluna K,Dagley LF,Sandow JJ,Kelly GL,Samson AL,Chappaz S,Khaw SL,Chang C,Morokoff A,Brinkmann K,Webb A,Hockings C,Hall CM,Kueh AJ,Ryan MT,Kluck RM,Bouillet P,Herold MJ,Gray DHD,Huang DCS,van Delft MF,Dewson G

Mon Nov 26 00:00:00 EST 2018

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.

Nature cell biology

Das A,Tanigawa S,Karner CM,Xin M,Lum L,Chen C,Olson EN,Perantoni AO,Carroll TJ

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); lipoprotein-associated phospholipase A2; PAF 2-acylhydrolase; PAF acetylhydrolase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma); Platelet-activating factor acetylhydrolase

Gene Aliases: LDL-PLA2; LP-PLA2; PAFAD; PAFAH; PLA2G7; R75400

UniProt ID: (Human) Q13093, (Mouse) Q60963

Entrez Gene ID: (Human) 7941, (Mouse) 27226

Molecular Function: protein modifying enzyme