Proteintech

PAK1 Polyclonal Antibody

PAK1 Antibody in Immunocytochemistry (ICC/IF)

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PAK1 Antibody (21401-1-AP) in ICC/IF

Immunofluorescent analysis of C6 cells using 21401-1-AP (PAK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PAK1 Antibody in Immunoprecipitation (IP)

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Product Details

21401-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PAK1 Fusion Protein Ag16102 (14-65 aa encoded by BC109299)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.67 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: APPMRNTST MIGAGSKDAG TLNHGSKPLP PNPEEKKKKD RFYRSILPGD KTN (14-65 aa encoded by BC109299)

Target Information

PAK1 is a serine/threonine kinase that is a target for restenosis, cancer, and immunosuppression treatment. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rac is required for the survival of cortical neurons.

Experimental neurology

Katayama KI,Zheng Y,Inoue N

Wed Mar 01 00:00:00 UTC 2023

Multi-proteomic Analysis Revealed Distinct Protein Profiles in Cerebrospinal Fluid of Patients Between Anti-NMDAR Encephalitis NORSE and Cryptogenic NORSE.

Molecular neurobiology

Wang D,Wu Y,Pan Y,Wang S,Liu G,Gao Y,Xu K

Sun Jan 01 00:00:00 UTC 2023

Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation.

PLoS pathogens

Wang DN,Ni JJ,Li JH,Gao YQ,Ni FJ,Zhang ZZ,Fang JY,Lu J,Yao YF

Wed Feb 01 00:00:00 UTC 2023

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

ACS applied materials & interfaces

Li Y,Yang L,Hu F,Xu J,Ye J,Liu S,Wang L,Zhuo M,Ran B,Zhang H,Ye J,Xiao J

Wed Jun 08 00:00:00 UTC 2022

SARS-CoV-2 hijacks macropinocytosis to facilitate its entry and promote viral spike-mediated cell-to-cell fusion.

The Journal of biological chemistry

Zhang YY,Liang R,Wang SJ,Ye ZW,Wang TY,Chen M,Liu J,Na L,Yang YL,Yang YB,Yuan S,Yin X,Cai XH,Tang YD

Tue Nov 01 00:00:00 UTC 2022

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Microbiology spectrum

Zhang B,Fan M,Fan J,Luo Y,Wang J,Wang Y,Liu B,Sun Y,Zhao Q,Hiscox JA,Nan Y,Zhou EM

Wed Feb 23 00:00:00 UTC 2022

LncRNA FAF attenuates hypoxia/ischaemia-induced pyroptosis via the miR-185-5p/PAK2 axis in cardiomyocytes.

Journal of cellular and molecular medicine

Gu J,Shi JZ,Wang YX,Liu L,Wang SB,Sun JT,Shan TK,Wang H,Wang QM,Wang LS

Sun May 01 00:00:00 UTC 2022

DOCK7 protects against replication stress by promoting RPA stability on chromatin.

Nucleic acids research

Gao M,Guo G,Huang J,Hou X,Ham H,Kim W,Zhao F,Tu X,Zhou Q,Zhang C,Zhu Q,Liu J,Yan Y,Xu Z,Yin P,Luo K,Weroha J,Deng M,Billadeau DD,Lou Z

Tue Apr 06 00:00:00 UTC 2021

Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway.

Acta pharmacologica Sinica

Liu CD,Liu NN,Zhang S,Ma GD,Yang HG,Kong LL,Du GH

Mon Mar 01 00:00:00 UTC 2021

Bu-Shen-Huo-Xue Decoction Ameliorates Diabetic Nephropathy by Inhibiting Rac1/PAK1/p38MAPK Signaling Pathway in High-Fat Diet/Streptozotocin-Induced Diabetic Mice.

Frontiers in pharmacology

Wang W,Long H,Huang W,Zhang T,Xie L,Chen C,Liu J,Xiong D,Hu W

Tue Dec 22 00:00:00 UTC 2020

P-21-activated kinase 1 contributes to tumor angiogenesis upon photodynamic therapy via the HIF-1α/VEGF pathway.

Biochemical and biophysical research communications

Jiang S,Gao Y,Yu QH,Li M,Cheng X,Hu SB,Song ZF,Zheng QC

Thu May 21 00:00:00 UTC 2020

NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway.

Gynecologic oncology

Xia P,Huang M,Zhang Y,Xiong X,Yan M,Xiong X,Yu W,Song E

Fri Feb 01 00:00:00 UTC 2019

NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway.

Gynecologic oncology

Xia P,Huang M,Zhang Y,Xiong X,Yan M,Xiong X,Yu W,Song E

Fri Feb 01 00:00:00 UTC 2019

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

Theranostics

Zhang M,Wang X,Guo F,Jia Q,Liu N,Chen Y,Yan Y,Huang M,Tang H,Deng Y,Huang S,Zhou Z,Zhang L,Zhang L

Thu Aug 20 00:00:00 UTC 2020

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

Theranostics

Zhang M,Wang X,Guo F,Jia Q,Liu N,Chen Y,Yan Y,Huang M,Tang H,Deng Y,Huang S,Zhou Z,Zhang L,Zhang L

Thu Aug 20 00:00:00 UTC 2020

Adenoviral vector-induced silencing of RGMa attenuates blood-brain barrier dysfunction in a rat model of MCAO/reperfusion.

Brain research bulletin

Li M,Wen Y,Zhang R,Xie F,Zhang G,Qin X

Sat Sep 01 00:00:00 UTC 2018

Adenoviral vector-induced silencing of RGMa attenuates blood-brain barrier dysfunction in a rat model of MCAO/reperfusion.

Brain research bulletin

Li M,Wen Y,Zhang R,Xie F,Zhang G,Qin X

Sat Sep 01 00:00:00 UTC 2018

Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis.

Cellular signalling

Zhang F,Lu YX,Chen Q,Zou HM,Zhang JM,Hu YH,Li XM,Zhang WJ,Zhang W,Lin C,Li XN

Tue Aug 01 00:00:00 UTC 2017

Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis.

Cellular signalling

Zhang F,Lu YX,Chen Q,Zou HM,Zhang JM,Hu YH,Li XM,Zhang WJ,Zhang W,Lin C,Li XN

Tue Aug 01 00:00:00 UTC 2017

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Scientific reports

Liang D,Wang Z,Yan Z,Hou S,Xu W,Wang L,Shang M,Qiao Z

Thu Mar 02 00:00:00 UTC 2017

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Scientific reports

Liang D,Wang Z,Yan Z,Hou S,Xu W,Wang L,Shang M,Qiao Z

Thu Mar 02 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: activated protein kinase alpha; ADRB2; alpha pak; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; kinase PAK1; MGC130000; MGC130001; p21 (CDKN1A)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK; p68-PAK; PAK 1; PAK-1; PAK1B; Protein kinase MUK2; Serine/threonine-protein kinase PAK 1; STE20 homolog, yeast

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Gene Aliases: AW045634; PAK-1; PAK1; Paka; PAKalpha

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UniProt ID: (Human) Q13153, (Rat) P35465

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Entrez Gene ID: (Human) 5058, (Mouse) 18479, (Rat) 29431

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Function(s)

protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding collagen binding ATP binding protein kinase binding nucleotide binding catalytic activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity

Process(es)

response to hypoxia positive regulation of protein phosphorylation protein phosphorylation exocytosis apoptotic process Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression phosphorylation dendrite development cell migration amygdala development signal transduction by protein phosphorylation actin cytoskeleton organization stress-activated protein kinase signaling cascade actin cytoskeleton reorganization activation of protein kinase activity cellular response to insulin stimulus regulation of actin cytoskeleton organization positive regulation of peptidyl-serine phosphorylation positive regulation of intracellular estrogen receptor signaling pathway wound healing regulation of apoptotic process receptor clustering regulation of MAPK cascade positive regulation of JUN kinase activity protein autophosphorylation branching morphogenesis of an epithelial tube neuron projection morphogenesis positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition dendritic spine development gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission cellular response to organic cyclic compound observational learning regulation of long-term synaptic potentiation MAPK cascade mitotic cell cycle stimulatory C-type lectin receptor signaling pathway axonogenesis positive regulation of cell proliferation positive regulation of cell migration T cell costimulation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ephrin receptor signaling pathway T cell receptor signaling pathway activation of MAPKK activity small GTPase mediated signal transduction intracellular signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PAK1 Polyclonal Antibody

PAK1 Antibody (21401-1-AP) in ICC/IF

Product Details

21401-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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