Proteintech

PAK1 Polyclonal Antibody

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PAK1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of C6 cells using 21401-1-AP (PAK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 21401-1-AP (PAK1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer slide using 21401-1-AP (PAK1 Antibody) at dilution of 1:50.

PAK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer slide using 21401-1-AP (PAK1 Antibody) at dilution of 1:50.

PAK1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PAK1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAK1 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAK1 Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAK1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAK1 Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAK1 Antibody in Western Blot (WB) — C6 cells were subjected to SDS PAGE followed by western blot with 21401-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PAK1 Antibody in Immunoprecipitation (IP) — IP result of anti-PAK1 (IP:21401-1-AP, 4ug; Detection:21401-1-AP 1:1000) with K-562 cells lysate 3200ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:10-1:100

-

Immunofluorescence (IF)

1:10-1:100

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Western Blot (WB)

1:500-1:2000

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PAK1 Fusion Protein Ag16102

Conjugate

Unconjugated

Form

Liquid

Concentration

0.67 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: activated protein kinase alpha; ADRB2; alpha pak; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; kinase PAK1; MGC130000; MGC130001; p21 (CDKN1A)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK; p68-PAK; PAK 1; PAK-1; PAK1B; Protein kinase MUK2; Serine/threonine-protein kinase PAK 1; STE20 homolog, yeast

Gene Aliases: AW045634; PAK-1; PAK1; Paka; PAKalpha

UniProt ID: (Human) Q32M54, (Rat) P35465

Entrez Gene ID: (Human) 5058, (Mouse) 18479, (Rat) 29431

response to hypoxia positive regulation of protein phosphorylation protein phosphorylation exocytosis apoptotic process Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression phosphorylation dendrite development cell migration amygdala development signal transduction by protein phosphorylation actin cytoskeleton organization stress-activated protein kinase signaling cascade actin cytoskeleton reorganization activation of protein kinase activity cellular response to insulin stimulus regulation of actin cytoskeleton organization positive regulation of peptidyl-serine phosphorylation positive regulation of intracellular estrogen receptor signaling pathway wound healing regulation of apoptotic process receptor clustering regulation of MAPK cascade positive regulation of JUN kinase activity protein autophosphorylation branching morphogenesis of an epithelial tube neuron projection morphogenesis positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition dendritic spine development gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission cellular response to organic cyclic compound observational learning regulation of long-term synaptic potentiation MAPK cascade mitotic cell cycle stimulatory C-type lectin receptor signaling pathway axonogenesis positive regulation of cell proliferation positive regulation of cell migration T cell costimulation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ephrin receptor signaling pathway T cell receptor signaling pathway activation of MAPKK activity small GTPase mediated signal transduction intracellular signal transduction

Process(es)

protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding collagen binding ATP binding protein kinase binding nucleotide binding catalytic activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity