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Invitrogen
PAK1 Polyclonal Antibody
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FIGURE: 1 / 13
PAK1 Antibody (71-9300) in ICC/IF
Immunofluorescence analysis of PAK-1 was done on 70% confluent log phase U87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PAK-1 Rabbit Polyclonal Antibody (Product # 71-9300) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at ... View More
Product Details
71-9300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rabbit
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from an internal sequence in the human PAK1 protein. This sequence is identical in human, mouse, and rat PAK1.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
PAK1 is a serine/threonine kinase that is a target for restenosis, cancer, and immunosuppression treatment. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activated protein kinase alpha; ADRB2; alpha pak; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; kinase PAK1; MGC130000; MGC130001; p21 (CDKN1A)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK; p68-PAK; PAK 1; PAK-1; PAK1B; protein kinase; Protein kinase MUK2; Serine/threonine-protein kinase PAK 1; STE20 homolog, yeast; unnamed protein product
Gene Aliases: alpha-PAK; AW045634; IDDMSSD; p65-PAK; PAK-1; PAK1; Paka; PAKalpha
UniProt ID: (Human) Q13153, (Rat) P35465
Entrez Gene ID: (Human) 5058, (Mouse) 18479, (Rat) 29431
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
protein binding
collagen binding
ATP binding
kinase activity
transferase activity
protein kinase binding
small GTPase binding
identical protein binding
gamma-tubulin binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
Rac GTPase binding
MAP kinase kinase kinase activity
response to hypoxia
positive regulation of protein phosphorylation
protein phosphorylation
exocytosis
apoptotic process
Rho protein signal transduction
regulation of mitotic cell cycle
neuromuscular junction development
metabolic process
response to organic substance
regulation of gene expression
phosphorylation
dendrite development
cell migration
amygdala development
signal transduction by protein phosphorylation
actin cytoskeleton organization
stress-activated protein kinase signaling cascade
actin cytoskeleton reorganization
activation of protein kinase activity
cellular response to insulin stimulus
regulation of actin cytoskeleton organization
positive regulation of peptidyl-serine phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
wound healing
regulation of apoptotic process
receptor clustering
regulation of MAPK cascade
positive regulation of JUN kinase activity
protein autophosphorylation
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
positive regulation of stress fiber assembly
negative regulation of cell proliferation involved in contact inhibition
dendritic spine development
gamma-aminobutyric acid secretion, neurotransmission
glutamate secretion, neurotransmission
cellular response to organic cyclic compound
observational learning
regulation of long-term synaptic potentiation
MAPK cascade
activation of MAPKK activity
mitotic cell cycle
small GTPase mediated signal transduction
axonogenesis
intracellular signal transduction
stimulatory C-type lectin receptor signaling pathway
chromatin remodeling
cellular response to DNA damage stimulus
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
cellular response to starvation
positive regulation of fibroblast migration
cerebellum development
establishment of cell polarity
positive regulation of cell migration
positive regulation of microtubule polymerization
Fc-gamma receptor signaling pathway involved in phagocytosis
positive regulation of axon extension
positive regulation of insulin receptor signaling pathway
hepatocyte growth factor receptor signaling pathway
ephrin receptor signaling pathway
regulation of axonogenesis
negative regulation of cell growth involved in cardiac muscle cell development
positive regulation of microtubule nucleation
positive regulation of protein targeting to membrane
regulation of actin filament organization
protein localization to cytoplasmic stress granule
positive regulation of vascular smooth muscle cell proliferation
positive regulation of vascular associated smooth muscle cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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