Invitrogen

PAK2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (23) PAK2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PAK2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PAK2 Polyclonal Antibody (Product # PA5-20254) at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

PAK2 Antibody in Western Blot (WB) — Knockdown of PAK2 was achieved by transfecting HeLa cells with PAK2 specific siRNAs (Silencer® select Product # S10023). Western blot analysis (Fig. a) was performed using modified membrane enriched cell extracts from the PAK2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PAK2 Polyclonal Antibody (Product # PA5-20254, 1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cyclin D1.

PAK2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PAK2 siRNA and decrease in signal was observed in Western Blot using Anti-PAK2 Polyclonal Antibody (Product # PA5-20254). {KD}

Product Details

PA5-20254

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	10 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A 14 amino acid peptide from near the amino terminus of human PAK2. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID	AB_2571863

Product Specific Information

A suggested positive control is Jurkat cell lysate.

PA5-20254 can be used with blocking peptide PEP-0390.

Target Information

PAK2 (serine/threonine-protein kinase PAK2) is a serine/threonine kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, or proliferation. It acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformation change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular Domains I and II of cell-surface glycoprotein CD44 mediate its trans-homophilic dimerization and tumor cluster aggregation.

The Journal of biological chemistry

Kawaguchi M,Dashzeveg N,Cao Y,Jia Y,Liu X,Shen Y,Liu H

PA5-20254 was used in Western Blotting to examine the biochemical properties of CD44 in homotypic tumor cell aggregation.

Fri Feb 28 00:00:00 EST 2020

Extracellular Domains I and II of cell-surface glycoprotein CD44 mediate its trans-homophilic dimerization and tumor cluster aggregation.

The Journal of biological chemistry

Kawaguchi M,Dashzeveg N,Cao Y,Jia Y,Liu X,Shen Y,Liu H

PA5-20254 was used in Western Blotting to examine the biochemical properties of CD44 in homotypic tumor cell aggregation.

Fri Feb 28 00:00:00 EST 2020

Bioinformatics

Protein Aliases: C-t-PAK2; gamma pak; Gamma-PAK; OPHN3; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p27; p34; p58; PAK alpha; PAK beta; PAK gamma; PAK-2; PAK-2p27; PAK-2p34; PAK3; PAK65; S6/H4 kinase; Serine/threonine-protein kinase PAK 2

Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; D16Ertd269e; gamma-PAK; mKIAA4182; PAK-2; PAK2; PAK65; PAKgamma

UniProt ID: (Human) Q13177, (Mouse) Q8CIN4, (Rat) Q64303

Entrez Gene ID: (Human) 5062, (Mouse) 224105, (Rat) 29432

Molecular Function: non-receptor serine/threonine protein kinase