Invitrogen

PAK2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

View all (19) PAK2 antibodies

Datasheet

Datasheet

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PAK2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of mouse spleen tissue using a PAK2 polyclonal antibody (Product # PA5-20254) at a 20 µg/mL dilution.

PAK2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of mouse spleen tissue using a PAK2 polyclonal antibody (Product # PA5-20254) at a 10 µg/mL dilution.

PAK2 Antibody in Western Blot (WB) — Knockdown of PAK2 was achieved by transfecting HeLa cells with PAK2 specific siRNAs (Silencer® select Product # S10023). Western blot analysis (Fig. a) was performed using modified membrane enriched cell extracts from the PAK2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PAK2 Polyclonal Antibody (Product # PA5-20254, 1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cyclin D1.

PAK2 Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PAK2 siRNA and decrease in signal was observed in Western Blot using Anti-PAK2 Polyclonal Antibody (Product # PA5-20254). {KD}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

20 µg/mL

Immunofluorescence (IF)

20 µg/mL

Immunohistochemistry (IHC)

10 µg/mL

Western Blot (WB)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 14 amino acid peptide from near the amino terminus of human PAK2.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_2571863

Product Specific Information

A suggested positive control is Jurkat cell lysate.

PA5-20254 can be used with blocking peptide PEP-0390.

Target Information

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-t-PAK2; Gamma-PAK; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p27; p34; p58; PAK-2; PAK-2p27; PAK-2p34; PAK65; PAK65, PAKgamma; S6/H4 kinase; Serine/threonine-protein kinase PAK 2

Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; D16Ertd269e; gamma-PAK; mKIAA4182; PAK-2; PAK2; PAK65; PAKgamma

UniProt ID: (Human) Q13177, (Mouse) Q8CIN4, (Rat) Q64303

Entrez Gene ID: (Human) 5062, (Mouse) 224105, (Rat) 29432

Function(s)